Three-stage repeated-batch immobilized cell fermentation to produce butanol from non-detoxified sugarcane bagasse hemicellulose hydrolysates

Author(s)

Chacon, Suranny Jimenez ^[1] ; Matias, Gabriela ^[1] ; Ezeji, Thaddeus Chukwuemeka ^[2] ; Maciel Filho, Rubens ^[1] ; Mariano, Adriano Pinto ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Sch Chem Engn, Lab Optimizat Design & Adv Control, Fermentat Div LOPCA Ferm, Campinas, SP - Brazil

^[2] Ohio State Univ, Ohio State Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH - USA

• Document type: Journal article
• Source: Bioresource Technology; v. 321, FEB 2021.
• Web of Science Citations: 0

Abstract

To enable the production of butanol with undiluted, non-detoxified sugarcane bagasse hemicellulose hydrolysates, this study developed a three-staged repeated-batch immobilized cell fermentation in which the efficiency of a 3D-printed nylon carrier to passively immobilize Clostridium saccharoperbutylacetonicum DSM 14923 was compared with sugarcane bagasse. The first stage consisted of sugarcane molasses fermentation, and in the second stage, non-detoxified sugarcane bagasse hemicellulose hydrolysates (SBHH) was pulse-fed to sugarcane molasses fermentation. In the next four batches, immobilized cells were fed with undiluted SBHH supplemented with molasses, and SBHH-derived xylose accounted for approximately 50% of the sugars. Bagasse was a superior carrier, and the average xylose utilization (33%) was significantly higher than the treatment with the 3D-printed carrier (16%). Notably, bagasse allowed for 43% of the butanol to be SBHH-derived. Overall, cell immobilization on lignocellulosic materials can be an efficient strategy to produce butanol from repeated-batch fermentation of non-detoxified hemicellulose hydrolysates. (AU)

• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/25925-0 - N-butanol production from fermentation of sugar cane bagasse hemicellulosic hydrolysate with cell immobilization
• Grantee: Gabriela Matias
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/23983-3 - Routes for production of sugarcane-based tires: efficient conversion of sugars to 1,3-butadiene precursors and techno-economic-environmental assessment
• Grantee: Adriano Pinto Mariano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants