Investigation of Ayahuasca beta-Carboline Alkaloids and Tryptamine in Sweat Samples from Religious Community Participants by GC-MS

Autor(es)

Tavares, Lidiane ^[1] ; Monedeiro, Fernanda ^[2] ; Bordin, Dayanne Mozaner ^{[1, 3]} ; De Martinis, Bruno Spinosa ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, 3900 Av Bandeirantes, BR-14050140 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, 3900 Av Bandeirantes, BR-14050140 Ribeirao Preto, SP - Brazil

^[3] Univ Technol Sydney, Sch Math & Phys Sci, 15 Broadway, Ultimo, NSW 2007 - Australia

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ANALYTICAL TOXICOLOGY; v. 44, n. 6, p. 601-609, JUL 2020.
• Citações Web of Science: 3

Resumo

Ayahuasca, a hallucinogenic beverage used in religious rituals in South America, has become a global phenomenon. Its main active components are the beta-carbolines alkaloids, harmine (HRM) and harmaline (HRL), as well as the potent hallucinogen N,N-dimethyltryptamine (DMT). Despite its rising consumption, information regarding possible clinical applications and toxicological effects of ayahuasca is still limited. This study presents the first investigation of the use of sweat for the determination of DMT, HRM and HRL in ayahuasca users during a religious ritual. Sweat is an alternative matrix with advantages over many conventional biological samples, mainly because the collection procedure is non-invasive, easy and simple and samples can be collected without disturbing the religious ritual. In the study, solid-phase extraction was performed under basic conditions. Linearity was observed ranging from 20 to 1500 ng/patch with coefficients of determination (R-2) higher than 0.99 for all analytes. The results indicated high selectivity for all investigated analytes, with extraction efficiency exceeding 70%, accuracy ranging from 87.5 to 102.4%, intra-assay precision of 1.85-9.44% and inter-assay precision between 3.34 and 9.85%. The limits of detection were 15 ng/patch for HRM and HRL and 10 ng/patch for DMT. The sweat proved to be a viable option to monitor ayahuasca use. (AU)

• Processo FAPESP: 12/00358-0 - Investigação de alcalóides beta-carbolinas, triptaminas em ayahuasca em amostras de suor, utilizando a extração em fase sólida modificada e cromatografia em fase gasosa acoplada a detector de nitrogênio-fósforo
• Beneficiário: Bruno Spinosa de Martinis
• Modalidade de apoio: Auxílio à Pesquisa - Regular