Oral administration ofBacillus subtilisandLactobacillus plantarummodulates the gut microbiota and increases the amylase activity of Nile tilapia (Oreochromis niloticus)

Autor(es)

Guimaraes, Mateus Cardoso ^{[1, 2]} ; Cavalcante da Silva Guimaraes, Amanda Iracy ^{[1, 2]} ; Natori, Mariene Miyoko ^[2] ; Fernandez Alarcon, Miguel Frederico ^[2] ; Dias, Danielle de Carla ^[2] ; Ishikawa, Carlos Massatoshi ^[2] ; Tapia-Paniagua, Silvana ^[3] ; Angel Morinigo, Miguel ^[3] ; Javier Moyano, Francisco ^[4] ; Tachibana, Leonardo ^[2]

Afiliação do(s) autor(es)

^[1] Fisheries Inst, Post Grad Program, Francisco Matarazzo Ave 455, BR-05001970 Sao Paulo, SP - Brazil

^[2] Fisheries Inst, APTA, SAA, Francisco Matarazzo Ave 455, BR-05001970 Sao Paulo, SP - Brazil

^[3] Malaga Univ, Dept Microbiol, Campus Teatinos S-N, Malaga 29071 - Spain

^[4] Almeria Univ, Dept Biol & Geol, Almeria - Spain

• Tipo de documento: Artigo Científico
• Fonte: AQUACULTURE INTERNATIONAL; v. 29, n. 1, p. 91-104, FEB 2021.
• Citações Web of Science: 0

Resumo

The aim of this research was to evaluate the possible positive effects of feeding Nile tilapia (Oreochromis niloticus) with a diet containing a commercial probiotic AQUA-PHOTO(R){[}Lactobacillus plantarum(1.51 x 10(6) CFU g(-1)) andBacillus subtilis(1.34 x 10(7) CFU g(-1))] on digestive enzymes' activity and gut microbiome profile. The experimental design was completely randomized, with two treatments {[}control and probiotic diets (0.08 g.100 g(-1)of feed)] and six replicate tanks per group (n = 8 animals per experimental unit, mean weight 8.18 g +/- 1.85 g). The 96 fish were distributed into 12 aquaria and fed with the probiotic and control diets for 7 days. After this period, intestine samples (anterior, middle, and posterior portions) were collected from two individuals per tank. The samples were analyzed for assessment of DGGE profile and digestive enzymes' activity (amylase, alkaline protease, and alkaline phosphatase). The results were subjected to analysis of variance (ANOVA) and the means compared byttest (P < 0.05). The addition ofB. subtilisandL. plantarumin the diets modulated the intestinal microbial biodiversity, more specifically the species richness and habitability index (P < 0.05). Besides, probiotic feeding also increased specific amylase activity in the anterior gut (P < 0.05). The other variables studied did not show any significant differences between treatments. The oral administration ofB. subtilisandL. plantarum(AQUA-PHOTO(R)) modulates intestinal microbiota profile and increases the amylase enzymatic activity. Therefore, this measure may provide better utilization of carbohydrate sources in the diet, supplying energy for Nile tilapia growth. (AU)

• Processo FAPESP: 13/09731-8 - Probióticos na alimentação de tilápia-do-Nilo, Oreochromis niloticus
• Beneficiário: Leonardo Tachibana
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/24599-7 - Possíveis alterações na microbiota intestinal promovida pela adição de probiótico Aqua-photo® em tilápia-do-Nilo (Oreochromis niloticus)
• Beneficiário: Amanda Iracy Cavalcante da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica
• Processo FAPESP: 16/13883-6 - Avaliação da atividade enzimática digestivas em tilápias-do-Nilo, Oreochromis niloticus, alimentadas com probiótico AQUA-PHOTO na dieta
• Beneficiário: Amanda Iracy Cavalcante da Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica