A photoelectrochemical enzyme biosensor based on functionalized hematite microcubes for rutin determination by square-wave voltammetry

Autor(es)

Mattos, Gabriel J. ^[1] ; Salamanca-Neto, Carlos A. R. ^[1] ; Barbosa, Eduardo C. M. ^[1] ; Camargo, Pedro H. C. ^[2] ; Dekker, Robert F. H. ^[3] ; Barbosa-Dekker, Aneli M. ; Sartori, Elen R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Ctr Ciencias Exatas, Dept Quim, Lab Eletroanalit & Sensores, Rodovia Celso Garcia Cid, PR 445, Km 380, CxP 10-011, BR-86057970 Londrina, Parana - Brazil

^[2] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Tecnol Fed Parana, Programa Posgrad Engn Ambiental, Campus Londrina, BR-86036370 Londrina, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchimica Acta; v. 188, n. 1 JAN 6 2021.
• Citações Web of Science: 0

Resumo

A photoelectrochemical biosensing strategy for the highly sensitive detection of the flavonoid rutin was developed by synergizing the photoelectrocatalytic properties of hematite (alpha-Fe2O3) decorated with palladium nanoparticles (PdNPs) and the biocatalysis towards laccase-based reactions. The integration of alpha-Fe2O3.PdNPs with a polyphenol oxidase as a biorecognition element yields a novel biosensing platform. Under visible light irradiation, the photoactive biocomposite can generate a stable photocurrent, which was found to be directly dependent upon the concentration of rutin. Under the optimal experimental conditions, the cathodic photocurrent, measured at 0.33 V vs. Ag/AgCl, from the square-wave voltammograms presented a linear dependence on the rutin concentration within the range of 0.008-30.0x10(-8) mol L-1 (sensitivity: 1.7 mu A.(x10(-8) M-1).cm(-2)), with an experimental detection limit (S/N=3) of 8.4x10(-11) mol L-1. The proposed biosensor device presented good selectivity towards rutin in the presence of various organic compounds and inorganic ions, demonstrating the potential application of this biosensing platform in complex matrices. This bioanalytical device also exhibited excellent operational and analytical properties, such as intra-day (standard deviation, SD=0.21%) and inter-day (SD=1.30%) repeatability, and long storage stability (SD=2.80% over 30 days).Graphical abstract (AU)

• Processo FAPESP: 15/26308-7 - Otimização das propriedades físico-químicas de materiais nano-estruturados e suas aplicações em reconhecimento molecular, catálise e conversão/armazenamento de energia
• Beneficiário: Roberto Manuel Torresi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/21366-9 - Materiais híbridos contendo nanopartículas metálicas para aplicações catalíticas
• Beneficiário: Pedro Henrique Cury Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11452-5 - Materiais híbridos compostos por óxidos e nanopartículas metálicas para aplicações catalíticas e fotocatalíticas
• Beneficiário: Eduardo César Melo Barbosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto