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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A photoelectrochemical enzyme biosensor based on functionalized hematite microcubes for rutin determination by square-wave voltammetry

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Author(s):
Mattos, Gabriel J. [1] ; Salamanca-Neto, Carlos A. R. [1] ; Barbosa, Eduardo C. M. [1] ; Camargo, Pedro H. C. [2] ; Dekker, Robert F. H. [3] ; Barbosa-Dekker, Aneli M. ; Sartori, Elen R. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Londrina, Ctr Ciencias Exatas, Dept Quim, Lab Eletroanalit & Sensores, Rodovia Celso Garcia Cid, PR 445, Km 380, CxP 10-011, BR-86057970 Londrina, Parana - Brazil
[2] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Tecnol Fed Parana, Programa Posgrad Engn Ambiental, Campus Londrina, BR-86036370 Londrina, Parana - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Microchimica Acta; v. 188, n. 1 JAN 6 2021.
Web of Science Citations: 0
Abstract

A photoelectrochemical biosensing strategy for the highly sensitive detection of the flavonoid rutin was developed by synergizing the photoelectrocatalytic properties of hematite (alpha-Fe2O3) decorated with palladium nanoparticles (PdNPs) and the biocatalysis towards laccase-based reactions. The integration of alpha-Fe2O3.PdNPs with a polyphenol oxidase as a biorecognition element yields a novel biosensing platform. Under visible light irradiation, the photoactive biocomposite can generate a stable photocurrent, which was found to be directly dependent upon the concentration of rutin. Under the optimal experimental conditions, the cathodic photocurrent, measured at 0.33 V vs. Ag/AgCl, from the square-wave voltammograms presented a linear dependence on the rutin concentration within the range of 0.008-30.0x10(-8) mol L-1 (sensitivity: 1.7 mu A.(x10(-8) M-1).cm(-2)), with an experimental detection limit (S/N=3) of 8.4x10(-11) mol L-1. The proposed biosensor device presented good selectivity towards rutin in the presence of various organic compounds and inorganic ions, demonstrating the potential application of this biosensing platform in complex matrices. This bioanalytical device also exhibited excellent operational and analytical properties, such as intra-day (standard deviation, SD=0.21%) and inter-day (SD=1.30%) repeatability, and long storage stability (SD=2.80% over 30 days).Graphical abstract (AU)

FAPESP's process: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage
Grantee:Roberto Manuel Torresi
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/21366-9 - HYBRID MATERIALS CONTAINING METAL NANOPARTICLES FOR CATALYTIC APPLICATIONS
Grantee:Pedro Henrique Cury Camargo
Support Opportunities: Regular Research Grants
FAPESP's process: 15/11452-5 - Hybrid materials composed of oxides and metallic nanoparticles for catalytic and photocatalytic applications
Grantee:Eduardo César Melo Barbosa
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)