Development of an electrochemical biosensor based on mesoporous silicon containing carbon nanotubes obtained by a sol-gel process. electrochemical determination of pesticides

• Grant number: 11/23047-7
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): June 01, 2012
• Effective date (End): February 22, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Sergio Antonio Spinola Machado
• Grantee: Thiago da Cruz Canevari
• Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

In this project the objective is related to the development of a biosensor based in the immobilization of acetilcholinesterase biomolecule in a mesoporous silicon matrix contaning carbon nanotubes, which will be used in the electrochemical determination of pesticides in foodstuffs. In the firs part of this project the non-ordered silica matrizes containing different amounts of nanotubes (10, 20 and 30%) will be synthesized by a Sol-Gel process using HF as the acid catalyst. In a second step, this silica matrixes will be used as substrate for the biomolecule immobilization. To that, the electrode will be immersed in a Britton-Robinson buffer solution containing the enzyme acetylcholinesterase allowing its adsorption by the modified surface. Changes in the structural properties of the surfaces will be studied. To do so, BET will be used to evaluate the surface area, transmission electronic microscopy will furnish the surface morphology, together with atomic force microscopy and scanning electron microscopy. Elemental analysis of C, H and N will determine the adsorption of enzyme on the modified surface. The electrochemical properties of the biosensor will be evaluated by electrochemical techniques such as cyclic voltammetry, pulses voltammetry, chronoamperometry, etc. The efficiency of the biosensor will be analysed by the inhibition caused by the carbamates and organophosphorous pesaticides in the acetylcholinesterase activity by hydrolises the neurotransmitter acetylthiocholine. The percentage of inhibition will be evaluated as a function of pesticide concentration and the analytical curve will be used to monitoring the pesticides concentrations in samples of cabbage, tomato, strawberry, etc.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CANEVARI, THIAGO C.; PRADO, THIAGO M.; CINCOTTO, FERNANDO H.; MACHADO, SERGIO A. S. Immobilization of ruthenium phthalocyanine on silica-coated multi-wall partially oriented carbon nanotubes: Electrochemical detection of fenitrothion pesticide. Materials Research Bulletin, v. 76, p. 41-47, APR 2016. Web of Science Citations: 18.

CINCOTTO, FERNANDO H.; CANEVARI, THIAGO C.; MACHADO, SERGIO A. S.; SANCHEZ, ALFREDO; RUIZ BARRIO, MARIA ASUNCION; VILLALONGA, REYNALDO; PINGARRON, JOSE M. Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol. Electrochimica Acta, v. 174, p. 332-339, AUG 20 2015. Web of Science Citations: 22.

RAZZINO, CLAUDIA A.; SGOBBI, LIVIA F.; CANEVARI, THIAGO C.; CANCINO, JULIANA; MACHADO, SERGIO A. S. Sensitive determination of carbendazim in orange juice by electrode modified with hybrid material. Food Chemistry, v. 170, p. 360-365, MAR 1 2015. Web of Science Citations: 33.

CINCOTTO, FERNANDO H.; CANEVARI, THIAGO C.; MACHADO, SERGIO A. S. Highly Sensitive Electrochemical Sensor for Determination of Vitamin D in Mixtures of Water-Ethanol. Electroanalysis, v. 26, n. 12, p. 2783-2788, DEC 2014. Web of Science Citations: 5.

CANEVARI, THIAGO C.; CINCOTTO, FERNANDO H.; LANDERS, RICHARD; MACHADO, SERGIO A. S. Synthesis and characterization of alpha-nickel (II) hydroxide particles on organic-inorganic matrix and its application in a sensitive electrochemical sensor for vitamin D determination. Electrochimica Acta, v. 147, p. 688-695, NOV 20 2014. Web of Science Citations: 10.

CINCOTTO, FERNANDO H.; CANEVARI, THIAGO C.; CAMPOS, ANDERSON M.; LANDERS, RICHARD; MACHADO, SERGIO A. S. Simultaneous determination of epinephrine and dopamine by electrochemical reduction on the hybrid material SiO2/graphene oxide decorated with Ag nanoparticles. ANALYST, v. 139, n. 18, p. 4634-4640, SEP 21 2014. Web of Science Citations: 28.

MALUTA, JAQUELINE R.; CANEVARI, THIAGO C.; MACHADO, SERGIO A. S. Sensitive determination of nitric oxide using an electrochemical sensor based on MWCNTs decorated with spherical Au nanoparticles. Journal of Solid State Electrochemistry, v. 18, n. 9, p. 2497-2504, SEP 2014. Web of Science Citations: 7.

CANEVARI, THIAGO C.; RAYMUNDO-PEREIRA, PAULO A.; LANDERS, RICHARD; BENVENUTTI, EDILSON V.; MACHADO, SERGIO A. S. Sol-gel thin-film based mesoporous silica and carbon nanotubes for the determination of dopamine, uric acid and paracetamol in urine. Talanta, v. 116, p. 726-735, NOV 15 2013. Web of Science Citations: 46.

CANEVARI, THIAGO C.; RAYMUNDO-PEREIRA, PAULO A.; LANDERS, RICHARD; MACHADO, SERGIO A. S. Direct Synthesis of Ag Nanoparticles Incorporated on a Mesoporous Hybrid Material as a Sensitive Sensor for the Simultaneous Determination of Dihydroxybenzenes Isomers. European Journal of Inorganic Chemistry, v. 2013, n. 33, p. 5746-5754, NOV 12 2013. Web of Science Citations: 16.