Low-Temperature Plasma as an Approach for Inhibiting a Multi-Species Cariogenic Biofilm

Autor(es)

Figueira, Leandro W. ^{[1, 2, 3]} ; Panariello, Beatriz H. D. ^[1] ; Koga-Ito, Cristiane Y. ^{[2, 3]} ; Duarte, Simone ^[1]

Afiliação do(s) autor(es)

^[1] Indiana Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN 46202 - USA

^[2] Sao Paulo State Univ, UNESP, Inst Sci & Technol, Dept Environm Engn, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Oral Biopathol Grad Program, Inst Sci & Technol, BR-12245000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APPLIED SCIENCES-BASEL; v. 11, n. 2 JAN 2021.
• Citações Web of Science: 0

Resumo

Featured Application Low exposure times of low-temperature plasma (LTP) affects single- and multi-species cariogenic biofilms, which makes it a promising candidate to contribute to the development of new protocols for the treatment and control of dental caries. This study aimed to determine how low-temperature plasma (LTP) treatment affects single- and multi-species biofilms formed by Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii formed on hydroxyapatite discs. LTP was produced by argon gas using the kINPen09 (TM) (Leibniz Institute for Plasma Science and Technology, INP, Greifswald, Germany). Biofilms were treated at a 10 mm distance from the nozzle of the plasma device to the surface of the biofilm per 30 s, 60 s, and 120 s. A 0.89% saline solution and a 0.12% chlorhexidine solution were used as negative and positive controls, respectively. Argon flow at three exposure times (30 s, 60 s, and 120 s) was also used as control. Biofilm viability was analyzed by colony-forming units (CFU) recovery and confocal laser scanning microscopy. Multispecies biofilms presented a reduction in viability (log(10) CFU/mL) for all plasma-treated samples when compared to both positive and negative controls (p < 0.0001). In single-species biofilms formed by either S. mutans or S. sanguinis, a significant reduction in all exposure times was observed when compared to both positive and negative controls (p < 0.0001). For single-species biofilms formed by S. gordonii, the results indicate total elimination of S. gordonii for all exposure times. Low exposure times of LTP affects single- and multi-species cariogenic biofilms, which indicates that the treatment is a promising source for the development of new protocols for the control of dental caries. (AU)

• Processo FAPESP: 19/01676-4 - Aplicação do plasma de baixa temperatura sob pressão atmosférica no controle de biofilmes cariogênicos
• Beneficiário: Leandro Wagner Figueira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/05856-7 - Aplicações de plasmas atmosféricos não-térmicos na odontologia: da bancada para a clínica
• Beneficiário: Cristiane Yumi Koga Ito
• Modalidade de apoio: Auxílio à Pesquisa - Temático