Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study

Autor(es)

Castro Goncalves, Francyenne Maira ^[1] ; Botazzo Delbem, Alberto Carlos ^[1] ; Gomes, Leonardo Fernandes ^[2] ; Emerenciano, Nayara Goncalves ^[1] ; Pessan, Juliano Pelim ^[1] ; Azevedo Romero, Giovana Dornelas ^[1] ; Cannon, Mark L. ^[3] ; Danelon, Marcelle ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Aracatuba Dept Prevent & Restorat Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Fed Univ Sao Carlos UFSCar, Dept Mat Engn, BR-13565905 Sao Carlos, SP - Brazil

^[3] Northwestern Univ, Ann & Robert Lurie Childrens Hosp, Feinberg Sch Med, Chicago, IL 60611 - USA

^[4] Univ Ribeirao Preto UNAERP, Sch Dent, BR-14096900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 122, FEB 2021.
• Citações Web of Science: 0

Resumo

Objective: To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with sodium trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus (R)), on the remineralization of dental enamel. Design: Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus (R) (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus (R), 4) 1100 F + MI Paste Plus (R) (1100 F-MI Paste Plus (R)), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus (R) (1100 F-TMP-MI Paste Plus (R)). Blocks were treated 2 x /day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus (R) for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (Delta KHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p < 0.001). Results: 1100 F-TMP-MI Paste Plus (R) group showed the best results of %SHR, Delta KHN and PLM (p < 0.001). F concentration was similar between the 1100 F, 1100 F-MI Paste Plus (R), and 1100 F-TMP-MI Paste Plus (R) groups (p > 0.001). 1100 F-TMP-MI Paste Plus (R) group showed the highest concentration of Ca and P in the enamel (p < 0.001). Conclusion: The association of 1100 F-TMP and MI Paste Plus (R) led to a significant increase in the remineralization of initial carious lesions. (AU)

• Processo FAPESP: 20/03506-6 - Efeito da associação de tratamentos com diferentes agentes remineralizantes contendo trimetafosfato de sódio e fosfopeptídeo de caseína-fosfato de cálcio amorfo em lesões iniciais de cárie: estudo in vitro
• Beneficiário: Natália Maria Castorino de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/21018-6 - Efeito da associação de tratamentos com fosfopeptídio de caseína-fosfato de cálcio amorfo e trimetafosfato de sódio sobre a remineralização de lesões de cárie: estudo in vitro
• Beneficiário: Giovana Dornelas Azevedo Romero
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/24761-4 - Efeito da associação de tratamentos com fosfopeptídeo de caseína-fosfato de cálcio amorfo e trimetafosfato de sódio sobre a a desmineralização e remineralização do esmalte: estudos in vitro
• Beneficiário: Francyenne Maira Castro Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Mestrado