Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood

Autor(es)

Marquez, Augusto ^[1] ; Santos, Moliria V. ^[2] ; Guirado, Gonzalo ^[3] ; Moreno, Alex ^[1] ; Aznar-Cervantes, Salvador D. ^[4] ; Cenis, Jose Luis ^[4] ; Santagneli, Silvia H. ^[5] ; Dominguez, Carlos ^[1] ; Omenetto, Fiorenzo G. ^{[6, 7, 8, 9]} ; Munoz-Berbel, Xavier ^[1]

Afiliação do(s) autor(es)

^[1] CSIC, CNM, Inst Microelect Barcelona IMB, Barcelona 08193 - Spain

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil

^[3] Univ Autonoma Barcelona, Dept Quim, Bellaterra 08193, Barcelona - Spain

^[4] Inst Murciano Invest & Desarrollo Agr & Alimentar, La Alberca 30150, Murcia - Spain

^[5] Sao Paulo State Univ UNESP, Inst Chem, BR-14801970 Araraquara, SP - Brazil

^[6] Tufts Univ, Dept Phys, Medford, MA 02155 - USA

^[7] Tufts Univ, Dept Biomed Engn, Silklab, Medford, MA 02155 - USA

^[8] Tufts Univ, Dept Elect & Comp Engn, Medford, MA 02155 - USA

^[9] Tufts Univ, Lab Living Devices, Medford, MA 02155 - USA

• Tipo de documento: Artigo Científico
• Fonte: LAB ON A CHIP; v. 21, n. 3, p. 608-615, FEB 7 2021.
• Citações Web of Science: 0

Resumo

In optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions, i.e. capillary pumping activity of 1.5 mm s(-1), capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays, e.g. immunoassays, with the aim of transferring them from central laboratories to the place of patient's care. (AU)

• Processo FAPESP: 18/11039-9 - Impressão 3D de fibroína de seda regenerada via litografia multifotônica para microfabricação de dispositivos fotônicos
• Beneficiário: Moliria Vieira dos Santos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/11591-8 - Microestruturação com lasers de femtossegundos em biopolímeros de celulose e fibroína: fabricação de dispositivos fotônicos
• Beneficiário: Moliria Vieira dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado