Peripheral Blood miRome Identified miR-155 as Potential Biomarker of MetS and Cardiometabolic Risk in Obese Patients

Autor(es):Mostrar menos -

Cerda, Alvaro ^{[1, 2, 3]} ; Amaral, Adonai Aralim ^[3] ; de Oliveira, Raquel ^[3] ; Moraes, Tamiris Invencioni ^[3] ; Braga, Aecio Assuncao ^[3] ; Graciano-Saldarriaga, Magda Elizabeth ^[3] ; Fajardo, Cristina Moreno ^[3] ; Hirata, Thiago Dominguez Crespo ^[3] ; Bonezi, Vivian ^[3] ; Campos-Salazar, Antony Brayan ^[3] ; Dorea, Egidio Lima ^[4] ; Bernik, Marcia Martins Silveira ^[4] ; Hirata, Mario Hiroyuki ^[3] ; Hirata, Rosario Dominguez Crespo ^[3]

Afiliação do(s) autor(es)

^[1] Univ La Frontera, CEMT BIOREN, Ctr Excellence Translat Med, Av Alemania 0458, Temuco 4810296 - Chile

^[2] Univ La Frontera, Dept Basic Sci, Av Alemania 0458, Temuco 4810296 - Chile

^[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Univ Hosp, Av Prof Lineu Prestes 2565, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 3 FEB 2021.
• Citações Web of Science: 0

Resumo

This study explored circulating miRNAs and target genes associated with metabolic syndrome (MetS) and cardiometabolic risk in obese patients. Small-RNA sequencing was used to assess the peripheral blood miRNome of 12 obese subjects (6 MetS and 6 non-MetS). Differentially expressed miRNAs and target genes were further analyzed by qPCR in a larger sample of obese patients (48 MetS and 32 non-MetS). miRNA:mRNA interactions were studied using in silico tools. miRNome analysis identified 10 downregulated miRNAs in MetS compared to non-Met patients (p < 0.05). In silico studies revealed three miRNAs (miR-155, miR-181a, and let-7a) and their predictive targets (CCAAT/enhancer-binding protein beta-CEBPB, KRAS proto-oncogene, GTPase-KRAS and suppressor of cytokine signaling 1-SOCS1) with a potential role in the insulin receptor signaling pathway. miR-155 expression was reduced and CEBPB mRNA levels were increased in MetS patients (p < 0.05), and these effects were correlated with the number of MetS diagnostic criteria (p < 0.05). Increased HOMA-IR (>7.6) was associated with low miR-155 levels, high CEBPB expression, and serum hsCRP (p < 0.05). miR-155 was negatively correlated with CEBPB, HOMA-IR, and plasma fibrinogen, and positively correlated with serum adiponectin (p < 0.05). Downregulation of circulating miR-155 is associated with insulin resistance, poor glycemic control, and increased MetS-related cardiometabolic risk, and these effects are potentially mediated by interaction with CEBPB. (AU)

• Processo FAPESP: 09/10069-2 - Influencia de marcadores genéticos sobre a resposta à intervenção dietética para redução do peso corporal
• Beneficiário: Rosario Dominguez Crespo Hirata
• Modalidade de apoio: Auxílio à Pesquisa - Regular