Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Peripheral Blood miRome Identified miR-155 as Potential Biomarker of MetS and Cardiometabolic Risk in Obese Patients

Full text
Author(s):
Show less -
Cerda, Alvaro [1, 2, 3] ; Amaral, Adonai Aralim [3] ; de Oliveira, Raquel [3] ; Moraes, Tamiris Invencioni [3] ; Braga, Aecio Assuncao [3] ; Graciano-Saldarriaga, Magda Elizabeth [3] ; Fajardo, Cristina Moreno [3] ; Hirata, Thiago Dominguez Crespo [3] ; Bonezi, Vivian [3] ; Campos-Salazar, Antony Brayan [3] ; Dorea, Egidio Lima [4] ; Bernik, Marcia Martins Silveira [4] ; Hirata, Mario Hiroyuki [3] ; Hirata, Rosario Dominguez Crespo [3]
Total Authors: 14
Affiliation:
[1] Univ La Frontera, CEMT BIOREN, Ctr Excellence Translat Med, Av Alemania 0458, Temuco 4810296 - Chile
[2] Univ La Frontera, Dept Basic Sci, Av Alemania 0458, Temuco 4810296 - Chile
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Univ Hosp, Av Prof Lineu Prestes 2565, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 3 FEB 2021.
Web of Science Citations: 0
Abstract

This study explored circulating miRNAs and target genes associated with metabolic syndrome (MetS) and cardiometabolic risk in obese patients. Small-RNA sequencing was used to assess the peripheral blood miRNome of 12 obese subjects (6 MetS and 6 non-MetS). Differentially expressed miRNAs and target genes were further analyzed by qPCR in a larger sample of obese patients (48 MetS and 32 non-MetS). miRNA:mRNA interactions were studied using in silico tools. miRNome analysis identified 10 downregulated miRNAs in MetS compared to non-Met patients (p < 0.05). In silico studies revealed three miRNAs (miR-155, miR-181a, and let-7a) and their predictive targets (CCAAT/enhancer-binding protein beta-CEBPB, KRAS proto-oncogene, GTPase-KRAS and suppressor of cytokine signaling 1-SOCS1) with a potential role in the insulin receptor signaling pathway. miR-155 expression was reduced and CEBPB mRNA levels were increased in MetS patients (p < 0.05), and these effects were correlated with the number of MetS diagnostic criteria (p < 0.05). Increased HOMA-IR (>7.6) was associated with low miR-155 levels, high CEBPB expression, and serum hsCRP (p < 0.05). miR-155 was negatively correlated with CEBPB, HOMA-IR, and plasma fibrinogen, and positively correlated with serum adiponectin (p < 0.05). Downregulation of circulating miR-155 is associated with insulin resistance, poor glycemic control, and increased MetS-related cardiometabolic risk, and these effects are potentially mediated by interaction with CEBPB. (AU)

FAPESP's process: 09/10069-2 - Influence of genetic makers on response to a dietary intervention to reduce body weight
Grantee:Rosario Dominguez Crespo Hirata
Support Opportunities: Regular Research Grants