Antimicrobial activity of effervescent denture tablets on multispecies biofilms

Autor(es)

Coimbra, Flavia C. T. ^[1] ; Rocha, Millena M. ^[1] ; Oliveira, Viviane C. ^[1] ; Macedo, Ana Paula ^[1] ; Pagnano, Valeria O. ^[1] ; Silva-Lovato, Claudia H. ^[1] ; Paranhos, Helena de F. O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Dent Mat & Prosthodont, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GERODONTOLOGY; v. 38, n. 1, p. 87-94, MAR 2021.
• Citações Web of Science: 1

Resumo

Purpose To investigate the impact of peroxide-based solutions in reducing viability and metabolic activity of multispecies biofilms on denture base acrylic resin surfaces and for removing them from these surfaces. Background Denture cleansers are effective in reducing monospecies biofilm; however, studies evaluating their action on multispecies biofilms are scarce. Materials and methods Sixty-nine denture base acrylic resin specimens (o 15 x 3 mm) were sterilised then contaminated withCandida albicans,Staphylococcus aureusandPseudomonas aeruginosato form multispecies biofilms. Biofilms were grown for 24 hours; subsequently, specimens were immersed in three different cleansing solutions (n = 9): nitradine (NI), fixodent (FX) and phosphate-buffered saline (Control), according to the respective manufacturer's instructions. After applying the hygiene protocols, viability of microorganisms was evaluated by counting colony-forming units and assessing metabolic activity. Moreover, biofilm removal capacity was estimated based on extension of cell-covered areas visualised in fluorescent microscopy micrographics. Results Microbial counts were solution-dependent; NI was effective against all microorganisms (P < .05). FX exhibited moderate antimicrobial action, reducingP aeruginosa(P < .05) andS aureus(P < .05) viability by approximately 2 logs. Both peroxide-based solutions reduced metabolic activity (P < .001) and biofilm-covered areas on specimen surfaces (P < .001). Conclusion Under the experimental conditions tested, these results demonstrated that peroxide-based solutions had favourable antimicrobial activity but promoted no broad elimination of aggregated multispecies biofilm. NI might be more suitable as complementary chemical agent for controlling multispecies denture biofilm. (AU)

• Processo FAPESP: 13/05494-1 - Avaliação in vitro da ação antimicrobiana de soluções higienizadoras de próteses totais
• Beneficiário: Helena de Freitas Oliveira Paranhos
• Modalidade de apoio: Auxílio à Pesquisa - Regular