Enzymatic routes to hydrogen and organic acids production from banana waste fermentation by autochthonous bacteria: Optimization of pH and temperature

Autor(es)

da Silva Mazareli, Raissa Cristina ^[1] ; Villa Montoya, Alejandra Carolina ^[1] ; Delforno, Tiago Palladino ^[2] ; Centurion, Victor Borin ^[2] ; de Oliveira, Valeria Maia ^[2] ; Silva, Edson Luiz ^[3] ; Amancio Varesche, Maria Bernadete ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, Lab Biol Proc, Campus 2, BR-13563120 Sao Carlos, SP - Brazil

^[2] Campinas Univ, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Campinas, SP - Brazil

^[3] Univ Fed Sao Carlos, Ctr Exact Sci & Technol, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 46, n. 12, p. 8454-8468, FEB 16 2021.
• Citações Web of Science: 0

Resumo

The Central Composite Rotational Design (CCRD) was employed to find the optimum pH (5.09-7.91) and temperature (27.1-46,9 degrees C) for hydrogen production in banana waste (BW) fermentation by autochthonous microbial biomass. The P and Rm ranged between 6.06 and 62.43 mL H2 and 1.13-12.56 mL H-2.h(-1), respectively. The temperature 37 degrees C and pH 7.0 were the optimum conditions for P (70.19 mL H-2) and Rm (12.43 mL H-2.h(-1)) as predicted by the mathematical model. Fructose and glucose are the primary alternative carbon sources in banana waste-fed batch reactors. The high concentration of lactic acid and H-2 production was associated to Lactobacillus (52-81%) and Clostridium (14-35%). However, the most important finding was about butyric acid (HBu). This acid is the better indicator of hydrogen production than acetic acid (HAc). The pH effected carbohydrates fermentation and organic acids production. The genes encoding the enzymes related to galactose, sucrose, fructose, arabinose and xylose metabolism were predominant. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 16/20048-6 - Aplicação de bactérias celulolíticas e fermentativas isoladas a partir de resíduos de bananas (Musa spp.) na obtenção de compostos de interesse biotecnológico.
• Beneficiário: Raissa Cristina da Silva Mazareli
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/06246-7 - Aplicação do conceito de biorrefinaria a estações de tratamento biológico de águas residuárias: o controle da poluição ambiental aliado à recuperação de matéria e energia
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático