Enzymatic routes to hydrogen and organic acids production from banana waste fermentation by autochthonous bacteria: Optimization of pH and temperature

Author(s)

da Silva Mazareli, Raissa Cristina ^[1] ; Villa Montoya, Alejandra Carolina ^[1] ; Delforno, Tiago Palladino ^[2] ; Centurion, Victor Borin ^[2] ; de Oliveira, Valeria Maia ^[2] ; Silva, Edson Luiz ^[3] ; Amancio Varesche, Maria Bernadete ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, Lab Biol Proc, Campus 2, BR-13563120 Sao Carlos, SP - Brazil

^[2] Campinas Univ, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Campinas, SP - Brazil

^[3] Univ Fed Sao Carlos, Ctr Exact Sci & Technol, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 46, n. 12, p. 8454-8468, FEB 16 2021.
• Web of Science Citations: 0

Abstract

The Central Composite Rotational Design (CCRD) was employed to find the optimum pH (5.09-7.91) and temperature (27.1-46,9 degrees C) for hydrogen production in banana waste (BW) fermentation by autochthonous microbial biomass. The P and Rm ranged between 6.06 and 62.43 mL H2 and 1.13-12.56 mL H-2.h(-1), respectively. The temperature 37 degrees C and pH 7.0 were the optimum conditions for P (70.19 mL H-2) and Rm (12.43 mL H-2.h(-1)) as predicted by the mathematical model. Fructose and glucose are the primary alternative carbon sources in banana waste-fed batch reactors. The high concentration of lactic acid and H-2 production was associated to Lactobacillus (52-81%) and Clostridium (14-35%). However, the most important finding was about butyric acid (HBu). This acid is the better indicator of hydrogen production than acetic acid (HAc). The pH effected carbohydrates fermentation and organic acids production. The genes encoding the enzymes related to galactose, sucrose, fructose, arabinose and xylose metabolism were predominant. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/20048-6 - Application cellulolytic and fermentative bacteria isolated from waste banana (Musa spp.) in obtainment of interest biotechnological compounds.
• Grantee: Raissa Cristina da Silva Mazareli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants