Assessment of physicochemical and phylogenetic features in the hydrogen production and Homoacetogenesis from wastes of coffee processing

• Grant number: 16/20047-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): January 01, 2017
• Effective date (End): March 31, 2019
• Field of knowledge: Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
• Principal researcher: Maria Bernadete Amâncio Varesche
• Grantee: Alejandra Carolina Villa Montoya
• Home Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery, AP.TEM

Abstract

The biological production of hydrogen (H2) is an interesting alternative by the possibility of using renewable biomass without generating polluting products. The coffee processing residues are attractive due to its abundance and low use in fermentation processes. However, low H2 yields are usually observed as a result of its consumption by bacteria or methanogenic archaea. Among them, hydrogen producing bacteria can change their metabolism to homoacetogenesis using the H2 as substrate to generate acetic acid. The mechanisms that lead to this metabolic change are still unclear. The objective of this study will be evaluate the effect of physical-chemical and microbiological parameters in H2 production and homoacetogenesis. To achieve this objective, two types of coffee processing waste will be tested: solid waste (husk) pretreated in hydrothermally reactor and wastewater from the wet coffee processing (including washing, depulping and degumming of the coffee fruits). The conditions of the hydrothermal pretreatment (temperature and time) will be selected by comparing highs and lows severities in the H2 production. Variables will be evaluated are pH (4,0, 5,5 and 7,0) and substrate (husk and wastewater) and substrate concentration (5, 15 and 25 g COD L -1 for the wastewater and 2, 4 and 6 g L-1 for the husk), by performing fermentation with mixtures of husk and wastewater. Reactors will be of 500 ml with 50 % headspace and 50 % of the reaction volume and temperature of 35°C. Fermentation will be natural, in order to encourage the development of microbiota from the wastes. Physical-chemical and chromatographic analyzes will be performed, such as chemical oxygen demand, solids, phenols, biogas composition, H2 production, sugars, organic acids and alcohols. The condition with highest H2 production and homoacetogenesis will be selected to perform phylogenetic characterization by sequencing the 16S rRNA (bacteria) and 18S (fungi) on a large scale (next generation).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILLA MONTOYA, ALEJANDRA CAROLINA; DA SILVA MAZARELI, RAISSA CRISTINA; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Improving the hydrogen production from coffee waste through hydrothermal pretreatment, co-digestion and microbial consortium bioaugmentation. BIOMASS & BIOENERGY, v. 137, JUN 2020. Web of Science Citations: 0.

VILLA MONTOYA, ALEJANDRA CAROLINA; DA SILVA MAZARELI, RAISSA CRISTINA; DELFORNO, TIAGO PALLADINO; CENTURION, VICTOR BORIN; DE OLIVEIRA, VALERIA MAIA; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Optimization of key factors affecting hydrogen production from coffee waste using factorial design and metagenomic analysis of the microbial community. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 45, n. 7, p. 4205-4222, FEB 7 2020. Web of Science Citations: 0.

VILLA MONTOYA, ALEJANDRA CAROLINA; DA SILVA MAZARELI, RAISSA CRISTINA; DELFORNO, TIAGO PALLADINO; CENTURION, VICTOR BONIN; SAKAMOTO, ISABEL KIMIKO; DE OLIVEIRA, VALERIA MAIA; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Hydrogen, alcohols and volatile fatty acids from the co-digestion of coffee waste (coffee pulp, husk, and processing wastewater) by applying autochthonous microorganisms. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 44, n. 39, p. 21434-21450, AUG 13 2019. Web of Science Citations: 0.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MONTOYA, Alejandra Carolina Villa. Evaluation of phyical-chemical and microbiological characteristics in hydrogen production and homoacetogenesis from coffee processing wastes. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de São Carlos São Carlos.