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Application cellulolytic and fermentative bacteria isolated from waste banana (Musa spp.) in obtainment of interest biotechnological compounds.

Grant number: 16/20048-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2017
Effective date (End): February 28, 2019
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Maria Bernadete Amâncio Varesche
Grantee:Raissa Cristina da Silva Mazareli
Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery, AP.TEM


Recently, the search of microbial consortia and isolates of cellulolytic and hydrogen producer bacteria from biomass has been target of study, with the goal of increasing the H2 and organic acids production. Considering the high generation of banana waste and the demand for renewable energy, it is required the development of sustainable technologies to reduce negative impacts on the environment and satisfy energy demand in the future. Therefore, the goal that project is the isolation of cellulolytic and fermentative bacteria from banana (Musa spp.), and the evaluation of their application as inoculum for the production of H2 and soluble metabolites in anaerobic batch reactor. In these tests will be used bananas (pulp and peel) as organic substrate. Factorial design 33 will be applied for analysis of the reactors, with variation in the substrate concentration (COD 5.0, 10 and 15 g/L), temperature (30°, 45° and 55°C) and pH (5.5, 6.5 and 7.5). Samples from batch reactors will be studied with physical and chemical analysis, such as pH, COD, total suspended solids (TSS) and volatile suspended solids (SSV), carbohydrates, sugars, phenols and organic acids. From the consortium obtained, will be performed the isolation of bacteria strains and their characterization using several carbon sources. PCR/DGGE and phylogenetic identification through 16S rRNA will be applied for the characterization of bacteria isolated and in the consortium.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA MAZARELI, RAISSA CRISTINA; VILLA MONTOYA, ALEJANDRA CAROLINA; DELFORNO, TIAGO PALLADINO; CENTURION, VICTOR BORIN; DE OLIVEIRA, VALERIA MAIA; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Enzymatic routes to hydrogen and organic acids production from banana waste fermentation by autochthonous bacteria: Optimization of pH and temperature. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 46, n. 12, p. 8454-8468, . (16/20048-6, 15/06246-7)
DA SILVA MAZARELI, RAISSA CRISTINA; SAKAMOTO, ISABEL KIMIKO; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Bacillus sp. isolated from banana waste and analysis of metabolic pathways in acidogenic systems in hydrogen production. Journal of Environmental Management, v. 247, p. 178-186, . (16/20048-6)
VILLA-MONTOYA, ALEJANDRA CAROLINA; DE OLIVEIRA, VALERIA MAIA. Metagenomic analysis of autochthonous microbial biomass from banana waste: Screening design of factors that affect hydrogen production. BIOMASS & BIOENERGY, v. 138, . (16/20048-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MAZARELI, Raissa Cristina da Silva. Optimization of hydrogen production from banana residue: evaluation of autochthonous bacteria diversity and functional distribution. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) São Carlos.

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