Recently, the search of microbial consortia and isolates of cellulolytic and hydrogen producer bacteria from biomass has been target of study, with the goal of increasing the H2 and organic acids production. Considering the high generation of banana waste and the demand for renewable energy, it is required the development of sustainable technologies to reduce negative impacts on the environment and satisfy energy demand in the future. Therefore, the goal that project is the isolation of cellulolytic and fermentative bacteria from banana (Musa spp.), and the evaluation of their application as inoculum for the production of H2 and soluble metabolites in anaerobic batch reactor. In these tests will be used bananas (pulp and peel) as organic substrate. Factorial design 33 will be applied for analysis of the reactors, with variation in the substrate concentration (COD 5.0, 10 and 15 g/L), temperature (30°, 45° and 55°C) and pH (5.5, 6.5 and 7.5). Samples from batch reactors will be studied with physical and chemical analysis, such as pH, COD, total suspended solids (TSS) and volatile suspended solids (SSV), carbohydrates, sugars, phenols and organic acids. From the consortium obtained, will be performed the isolation of bacteria strains and their characterization using several carbon sources. PCR/DGGE and phylogenetic identification through 16S rRNA will be applied for the characterization of bacteria isolated and in the consortium.
News published in Agência FAPESP Newsletter about the scholarship: