Simulated microgravity accelerates aging in Saccharomyces cerevisiae

Autor(es)

Montanari Fukuda, Ana Paula ^[1] ; Camandona, Vittoria de Lima ^[1] ; Mendonca Francisco, Kelliton Jose ^[1] ; Rios-Anjos, Rafaela Maria ^[1] ; do Lago, Claudimir Lucio ^[2] ; Ferreira-Junior, Jose Ribamar ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIFE SCIENCES IN SPACE RESEARCH; v. 28, p. 32-40, FEB 2021.
• Citações Web of Science: 0

Resumo

The human body experiences physiological changes under microgravity environment that phenocopy aging on Earth. These changes include early onset osteoporosis, skeletal muscle atrophy, cardiac dysfunction, and immunosenescence, and such adaptations to the space environment may pose some risk to crewed missions to Mars. To investigate the effect of microgravity on aging, many model organisms have been used such as the nematode Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and mice. Herein we report that the budding yeast Saccharomyces cerevisiae show decreased replicative lifespan (RLS) under simulated microgravity in a clinostat. The reduction of yeast lifespan is not a result of decreased tolerance to heat shock or oxidative stress and could be overcome either by deletion of FOB1 or calorie restriction, two known interventions that extend yeast RLS. Deletion of the sirtuin gene SIR2 worsens the simulated microgravity effect on RLS, and together with the fob1 Delta mutant phenotype, it suggests that simulated microgravity augments the formation of extrachromosomal rDNA circles, which accumulate in yeast during aging. We also show that the chronological lifespan in minimal medium was not changed when cells were grown in the clinostat. Our data suggest that the reduction in longevity due to simulated microgravity is conserved in yeast, worms, and flies, and these findings may have potential implications for future crewed missions in space, as well as the use of microgravity as a model for human aging. (AU)

• Processo FAPESP: 17/09938-2 - Análise do efeito da superexpressão de HSP27 humana na longevidade de Saccharomyces cerevisiae
• Beneficiário: José Ribamar dos Santos Ferreira Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular