Preparation, characterization and in vitro anticancer performance of nanoconjugate based on carbon quantum dots and 5-Fluorouracil

Autor(es)

Cutrim, Elaine S. M. ^[1] ; Vale, Andre A. M. ^[2] ; Manzani, Danilo ^[3] ; Barud, Hernane S. ^[4] ; Rodriguez-Castellon, Enrique ^[5] ; Santos, Ana P. S. A. ^[2] ; Alcantara, Ana C. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Maranhao, Dept Chem, Hybrid & Bionanocomposite Mat Res Grp Bionanos, BR-65080805 Sao Luis, Maranhao - Brazil

^[2] Univ Fed Maranhao, Biol & Hlth Sci Ctr, Lab Appl Canc Immunol, BR-65080805 Sao Luis, Maranhao - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13566590 Sao Carlos, SP - Brazil

^[4] Univ Araraquara Uniara, Lab Biopolimeros & Biomat, BR-14801320 Araraquara, SP - Brazil

^[5] Univ Malaga, Fac Ciencias, Dept Quim Inorgan, E-29071 Malaga - Spain

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 120, JAN 2021.
• Citações Web of Science: 0

Resumo

This study is focused on the development of a nanodevice for loading and release of 5-Fluorouracil (5-FU) with a view to improving its therapeutic efficiency, using as strategy the fabrication of a nanoconjugate through drug anchorage on the surface of carbon quantum dots (CQD). Several physicochemical and analytical techniques were employed to obtain information about materials morphology, structure, and optical properties. The results indicated that the interactions between both entities resulted in good physicochemical properties and photo stability. Acid pH favored drug release, indicating a tendency to release 5-FU from 5-FU-CQD into the tumor microenvironment. The cytotoxicity of CQD and 5-FU-CQD nanoconjugate was evaluated against normal human lung fibroblast (GM07492A) and human breast cancer (MCF-7) cell lines. The CQD was non-toxic, indicating that these materials are biocompatible and can be used as a nanocarrier for 5-FU in biological systems. For the 5-FUCQD nanoconjugate, it was observed a reduction in toxicity for normal cells compared to free 5-FU, suggesting that drug anchoring in CQD reduced drug-associated toxicity, while for cancer cells exhibited an antitumor effect equivalent to that of the free drug, opening perspectives for the application of this material in anticancer therapy. (AU)

• Processo FAPESP: 18/16126-7 - Vidros opticamente ativos para aplicações fotônicas: síntese, caracterização e aplicação de vidros óxidos contendo quantum dots.
• Beneficiário: Danilo Manzani
• Modalidade de apoio: Auxílio à Pesquisa - Regular