Gut microbiota comparison of vaginally and cesarean born infants exclusively breastfed by mothers secreting alpha 1-2 fucosylated oligosaccharides in breast milk

Autor(es)

Tonon, Karina M. ^[1] ; Morais, Tania B. ^[2] ; Taddei, Carla R. ^{[3, 4]} ; Araujo-Filho, Humberto B. ^[5] ; Abrao, V, Ana Cristina F. ; Miranda, Antonio ^[6] ; de Morais, Mauro B. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Nutr Postgrad Program, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Food Qual Control Lab, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Div Pediat Gastroenterol, Sao Paulo - Brazil

^[6] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 2 FEB 8 2021.
• Citações Web of Science: 2

Resumo

Background Exclusive breastfeeding promotes beneficial modifications on the microbiota of cesarean born infants, but little is known about the role of specific breast milk components in this modulation. Women with an active FUT2 gene (called secretors) secrete alpha 1-2 fucosylated human milk oligosaccharides (HMOs), which promote Bifidobacterium in the infant's gut and may modulate the microbiota of cesarean born infants. Objective To compare the microbiota composition of cesarean and vaginally born infants breastfed by secretor mothers. Methods Maternal secretor status was determined by the occurrence of 4 different alpha 1-2 fucosylated HMOs in breast milk by LC-MS. The fecal microbiota composition from cesarean and vaginally born infants was analyzed by 16S rRNA gene sequencing and qPCR, stratified by the maternal secretor status, and compared. Results Alpha and beta diversity were not significantly different in cesarean born, secretor-fed infants (CSe+) compared to vaginally born, secretor-fed infants (VSe+). There were no significant differences in the fecal relative abundance of Bifidobacterium between CSe+ and VSe+ infants, but the prevalence of the species B. longum was lower in CSe+. The fecal relative abundance of Bacteroides was also lower, while Akkermansia and Kluyvera were higher in CSe+ infants. Conclusion Cesarean and vaginally born infants fed with breast milk containing the alpha 1-2 fucosylated HMOs fraction present similar amounts of Bifidobacterium in the feces, but differences are observed in other members of the microbiota. (AU)

• Processo FAPESP: 14/13514-5 - Oligossacarídeos do leite humano: relação com as características socioambientais, genéticas, presença de doença alérgica materna e com a microbiota do lactente
• Beneficiário: Tania Beninga de Morais
• Modalidade de apoio: Auxílio à Pesquisa - Regular