Engineering 3D printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration

Autor(es)

Backes, Eduardo H. ^{[1, 2]} ; Fernandes, Emanuel M. ^{[3, 2]} ; Diogo, Gabriela S. ^{[3, 2]} ; Marques, Catarina F. ^{[3, 2]} ; Silva, Tiago H. ^{[3, 2]} ; Costa, Lidiane C. ^[1] ; Passador, Fabio R. ^[4] ; Reis, Rui L. ^{[3, 2]} ; Pessan, Luiz A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Grad Program Mat Sci & Engn, Via Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Minho, Headquarters European Inst Excellence Tissue Engn, I3Bs Res Inst Biomat Biodegradables & Biomi, 3Bs Res Grp, AvePk, Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes - Portugal

^[3] ICVS 3Bs PT Govt Associate Lab, Braga, Guimaraes - Portugal

^[4] Univ Fed Sao Paulo, Sci & Technol Inst, Talim St 330, BR-12231280 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 122, MAR 2021.
• Citações Web of Science: 0

Resumo

In this study, polylactic acid (PLA) filled with hydroxyapatite (HA) or beta-tricalcium phosphate (TCP) in 5 wt% and 10 wt% of concentration were produced employing twin-screw extrusion followed by fused filament fabrication in two different architectures, varying the orientation of fibers of adjacent layers. The extruded 3D filaments presented suitable rheological and thermal properties to manufacture of 3D scaffolds envisaging bone tissue engineering. The produced scaffolds exhibited a high level of printing accuracy related to the 3D model; confirmed by micro-CT and electron microscopy analysis. The developed architectures presented mechanical properties compatible with human bone replacement. The addition of HA and TCP made the filaments bioactive, and the deposition of new calcium phosphates was observed upon 7 days of incubation in simulated body fluid, exemplifying a microenvironment suitable for cell attachment and proliferation. After 7 days of cell culture, the constructs with a higher percentage of HA and TCP demonstrated a significantly superior amount of DNA when compared to neat PLA, indicating that higher concentrations of HA and TCP could guide a good cellular response and increasing cell cytocompatibility. Differentiation tests were performed, and the biocomposites of PLA/HA and PLA/TCP exhibited earlier markers of cell differentiation as confirmed by alkaline phosphatase and alizarin red assays. The 3D printed composite scaffolds, manufactured with bioactive materials and adequate porous size, supported cell attachment, proliferation, and differentiation, which together with their scalability, promise a high potential for bone tissue engineering applications. (AU)

• Processo FAPESP: 17/11366-7 - Desenvolvimento de scaffolds bioinspirados de PLA/biovidros via impressão 3D
• Beneficiário: Eduardo Henrique Backes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21313-1 - Estudo da cinética de reação e da adição de desativadores de metais na graftização de anidrido maleico em polipropileno, na presença e ausência de nanopartículas
• Beneficiário: Silvia Helena Prado Bettini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/13625-2 - Avaliação das propriedades mecânicas e da bioatividade de scaffolds de PLA/cargas bioativas bioinspirados produzidos via impressão 3d
• Beneficiário: Eduardo Henrique Backes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 17/09609-9 - Desenvolvimento de scaffolds bioinspirados de PLA/cargas cerâmicas bioativas via impressão 3D
• Beneficiário: Luiz Antonio Pessan
• Modalidade de apoio: Auxílio à Pesquisa - Regular