Photobiomodulation therapy drives massive epigenetic histone modifications, stem cells mobilization and accelerated epithelial healing

Autor(es)

Martins, Manoela D. ^{[1, 2, 3]} ; Silveira, Felipe Martins ^[1] ; Martins, Marco A. T. ^{[3, 4]} ; Almeida, Luciana O. ^[5] ; Bagnato, Vanderlei S. ^[6] ; Squarize, Cristiane H. ^[2] ; Castilho, Rogerio M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba - Brazil

^[2] Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Lab Epithelial Biol, Ann Arbor, MI 48109 - USA

^[3] Univ Fed Rio Grande do Sul, Sch Dent, Dept Oral Pathol, Porto Alegre, RS - Brazil

^[4] Univ Fed Rio Grande do Sul, Hosp Clin Porto Alegre HCPA UFRGS, Dept Oral Med, Porto Alegre, RS - Brazil

^[5] Univ Sao Paulo, Sch Dent, Dept Basic & Oral Biol, Lab Tissue Culture, Ribeirao Preto, RS - Brazil

^[6] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. 14, n. 2 FEB 2021.
• Citações Web of Science: 0

Resumo

Emerging evidence indicates the clinical benefits of photobiomodulation therapy (PBMT) in the management of skin and mucosal wounds. Here, we decided to explore the effects of different regiments of PBMT on epithelial cells and stem cells, and the potential implications over the epigenetic circuitry during healing. Scratch-wound migration, immunofluorescence (anti-acetyl-Histone H3, anti-acetyl-CBP/p300 and anti-BMI1), nuclear morphometry and western blotting (anti-Phospho-S6, anti-methyl-CpG binding domain protein 2 {[}MBD2]) were performed. Epithelial stem cells were identified by the aldehyde dehydrogenase enzymatic levels and sphere-forming assay. We observed that PBMT-induced accelerated epithelial migration and chromatin relaxation along with increased levels of histones acetylation, the transcription cofactors CBP/p300 and mammalian target of rapamycin. We further observed a reduction of the transcription repression-associated protein MBD2 and a reduced number of epithelial stem cells and spheres. In this study, we showed that PBMT could induce epigenetic modifications of epithelial cells and control stem cell fate, leading to an accelerated healing phenotype. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs