Early immune responses and parasite tissue distribution in mice experimentally infected with oocysts of either archetypal or non-archetypal genotypes of Toxoplasma gondii

Autor(es)

Chiebao, Daniela P. ^[1] ; Bartley, Paul M. ^[2] ; Chianini, Francesca ^[2] ; Black, Lauren E. ^[2] ; Burrells, Alison ^[2] ; Pena, Hilda F. J. ^[1] ; Soares, Rodrigo M. ^[1] ; Innes, Elisabeth A. ^[2] ; Katzer, Frank ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci FMVZ, Dept Prevent Vet Med, 87 Prof Doutor Orlando Marques de Paiva Ave, BR-05508270 Sao Paulo - Brazil

^[2] Moredun Res Inst, Pentland Sci Pk, Edinburgh EH26 0PZ, Midlothian - Scotland

• Tipo de documento: Artigo Científico
• Fonte: Parasitology; v. 148, n. 4, p. 464-476, APR 2021.
• Citações Web of Science: 0

Resumo

In most of the world Toxoplasma gondii is comprised of archetypal types (types I, II and III); however, South America displays several non-archetypal strains. This study used an experimental mouse model to characterize the immune response and parasite kinetics following infection with different parasite genotypes. An oral inoculation of 50 oocysts per mouse from T. gondii M4 type II (archetypal, avirulent), BrI or BrIII (non-archetypal, virulent and intermediate virulent, respectively) for groups (G)2, G3 and G4, respectively was used. The levels of mRNA expression of cytokines, immune compounds, cell surface markers and receptor adapters {[}interferon gamma (IFN gamma), interleukin (IL)-12, CD8, CD4, CD25, CXCR3 and MyD88] were quantified by SYBR green reverse transcription-quantitative polymerase chain reaction. Lesions were characterized by histology and detection by immunohistochemistry established distribution of parasites. Infection in G2 mice was mild and characterized by an early MyD88-dependent pathway. In G3, there were high levels of expression of pro-inflammatory cytokines IFN gamma and IL-12 in the mice showing severe clinical symptoms at 8-11 days post infection (dpi), combined with the upregulation of CD25, abundant tachyzoites and tissue lesions in livers, lungs and intestines. Significant longer expression of IFN gamma and IL-12 genes, with other Th1-balanced immune responses, such as increased levels of CXCR3 and MyD88 in G4, resulted in survival of mice and chronic toxoplasmosis, with the occurrence of tissue cysts in brain and lungs, at 14 and 21 dpi. Different immune responses and kinetics of gene expression appear to be elicited by the different strains and non-archetypal parasites demonstrated higher virulence. (AU)

• Processo FAPESP: 11/19297-8 - Estudo da infecção oral por Toxoplasma gondii em ovinos: avaliação da transmissão congênita em infecções experimentais por meio de diferentes linhagens brasileiras
• Beneficiário: Rodrigo Martins Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular