Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation

Autor(es)

Mamana, Ana Carolina ^[1] ; Stincarelli, Maria ^[2] ; Sarmento, Dmitry Jose De Santana ^[3] ; Mendes Batista, Alexandre ^[1] ; Tozetto-Mendoza, Tania Regina ^[1] ; Gallottini, Marina ^[3] ; De Abreu Pestana, Jose Osmar Medina ^[4] ; Braz-Silva, Paulo Henrique ^{[3, 1]} ; Giannecchini, Simone ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Trop Med Sao Paulo, Sch Med, Lab Virol, Sao Paulo - Brazil

^[2] Univ Florence, Dept Expt & Clin Med, Florence - Italy

^[3] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Kidney & Hypertens Hosp, Div Renal Transplantat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL MICROBIOLOGY; v. 13, n. 1 JAN 1 2021.
• Citações Web of Science: 0

Resumo

Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence. Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity. Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR. Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation. Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation. (AU)

• Processo FAPESP: 15/07727-9 - Avaliação do ambiente imunoregulatório de lesões potencialmente malignas de boca, carcinomas de boca e orofaringe relacionados ou não ao HPV.
• Beneficiário: Paulo Henrique Braz da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular