| Full text | |
| Author(s): |
Mamana, Ana Carolina
[1]
;
Stincarelli, Maria
[2]
;
Sarmento, Dmitry Jose De Santana
[3]
;
Mendes Batista, Alexandre
[1]
;
Tozetto-Mendoza, Tania Regina
[1]
;
Gallottini, Marina
[3]
;
De Abreu Pestana, Jose Osmar Medina
[4]
;
Braz-Silva, Paulo Henrique
[3, 1]
;
Giannecchini, Simone
[2]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Inst Trop Med Sao Paulo, Sch Med, Lab Virol, Sao Paulo - Brazil
[2] Univ Florence, Dept Expt & Clin Med, Florence - Italy
[3] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Kidney & Hypertens Hosp, Div Renal Transplantat, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF ORAL MICROBIOLOGY; v. 13, n. 1 JAN 1 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence. Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity. Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR. Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation. Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation. (AU) | |
| FAPESP's process: | 15/07727-9 - Evaluation of immunoregulatory microenvironment of oral premalignant lesions, squamous cell carcinomas and oropharyngeal carcinomas related to HPV or not. |
| Grantee: | Paulo Henrique Braz da Silva |
| Support Opportunities: | Regular Research Grants |