Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows

Autor(es):Mostrar menos -

Mazzarella, Rosane ^[1] ; Bastos, Natalia Marins ^[1] ; Bridi, Alessandra ^[1] ; del Collado, Maite ^[1] ; Andrade, Gabriella Mamede ^[1] ; Pinzon, Jorge ^[2] ; Prado, Cibele Maria ^[1] ; Silva, Luciano Andrade ^[1] ; Meirelles, Flavio Vieira ^[1] ; Pugliesi, Guilherme ^[3] ; Perecin, Felipe ^[1] ; da Silveira, Juliano Coelho ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Anim Sci & Food Engn, Mol Morphol & Dev Lab, Dept Vet Med, Pirassununga - Brazil

^[2] Univ Nacl Colombia, Fac Vet Med & Anim Sci, Grad Dept, Bogota - Colombia

^[3] Univ Sao Paulo, Mol Endocrinol Physiol Lab, Dept Anim Reprod, Sch Vet Med & Anim Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN VETERINARY SCIENCE; v. 8, MAR 4 2021.
• Citações Web of Science: 3

Resumo

Early embryonic development occurs in the oviduct, where an ideal microenvironment is provided by the epithelial cells and by the oviductal fluid produced by these cells. The oviductal fluid contains small extracellular vesicles (sEVs), which through their contents, including microRNAs (miRNAs), can ensure proper cell communication between the mother and the embryo. However, little is known about the modulation of miRNAs within oviductal epithelial cells (OECs) and sEVs from the oviductal fluid in pregnant cows. In this study, we evaluate the miRNAs profile in sEVs from the oviductal flushing (OF-sEVs) and OECs from pregnant cows compared to non-pregnant, at 120 h after ovulation induction. In OF-sEVs, eight miRNAs (bta-miR-126-5p, bta-miR-129, bta-miR-140, bta-miR-188, bta-miR-219, bta-miR-345-3p, bta-miR-4523, and bta-miR-760-3p) were up-regulated in pregnant and one miRNA (bta-miR-331-5p) was up-regulated in non-pregnant cows. In OECs, six miRNAs (bta-miR-133b, bta-miR-205, bta-miR-584, bta-miR-551a, bta-miR-1193, and bta-miR-1225-3p) were up-regulated in non-pregnant and none was up-regulated in pregnant cows. Our results suggest that embryonic maternal communication mediated by sEVs initiates in the oviduct, and the passage of gametes and the embryo presence modulate miRNAs contents of sEVs and OECs. Furthermore, we demonstrated the transcriptional levels modulation of selected genes in OECs in pregnant cows. Therefore, the embryonic-maternal crosstalk potentially begins during early embryonic development in the oviduct through the modulation of miRNAs in OECs and sEVs in pregnant cows. (AU)

• Processo FAPESP: 15/21829-9 - EMU concedido no projeto 2014/22887-0: NanoSight LM10
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 19/04981-2 - Efeitos da suplementação de vesículas extracelulares durante a clivagem de embriões bovinos
• Beneficiário: Rosane Mazzarella
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores