Genotoxicity induced by nerol, an essential oil present in citric plants using human peripheral blood mononuclear cells (PBMC) and HepG2/C3A cells as a model

Autor(es)

Silva, Brian Ogushi ^[1] ; Orlando, Juliana Botinhon ^[1] ; Pires, Camila Lehnhardt ^[2] ; Hiruma-Lima, Clelia Akiko ^[2] ; de Mascarenhas Gaivao, Isabel ^{[3, 4]} ; Perazzo, Fabio Ferreira ^[5] ; Maistro, Edson Luis ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Fac Philosophy & Sci, Speech & Hearing Therapy Dept, Marilia, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Programa Posgrad Biol Geral & Aplicada, Inst Biociencias, Botucatu, SP - Brazil

^[3] Univ Tras Os Montes & Alto Douro UTAD, Dept Genet & Biotechnol, Vila Real - Portugal

^[4] Univ Tras Os Montes & Alto Douro UTAD, Anim & Vet Res Ctr CECAV, Vila Real - Portugal

^[5] Fed Univ Sao Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci, Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 84, n. 12 MAR 2021.
• Citações Web of Science: 0

Resumo

Nerol (cis-3,7-dimethyl-2,6-octadien-1-ol) is a monoterpene widely used in cosmetic products, household detergents and cleaners, as well as a flavoring in several food products. Despite the high level of human exposure to nerol, an absence of studies regarding potential genetic toxicity in human cells exists. The aim of this investigation was to examine the cytotoxic and genotoxic potential of this monoterpene on human peripheral blood mononuclear cells as well as hepatic metabolizing HepG2/C3A human cell line. Cytotoxicity was assessed using trypan blue staining and MTT assay while genotoxicity was determined utilizing the comet and micronucleus test. Cytotoxicity tests showed cell viability greater than 70% for concentrations between 2.5 and 500 mu g/ml. Both cell types exhibited significant DNA damage and chromosomal mutations after medium and high concentration incubation with nerol indicating that the safety of use of this monoterpene in various formulations to which humans are exposed needs to be monitored and requires more comprehensive investigations. (AU)

• Processo FAPESP: 17/24149-4 - Estudo da citotoxicidade, genotoxicidade, monitoramento do ciclo celular, indução de apoptose e expressão gênica, dos extratos de folhas e caules de Rubus rosaefolius em células humanas em cultura.
• Beneficiário: Edson Luis Maistro
• Modalidade de apoio: Auxílio à Pesquisa - Regular