Sustainable Strategy Based on Induced Precipitation for the Purification of Phycobiliproteins

Autor(es)

Martins, Margarida ^[1] ; Soares, Bruna P. ^[1] ; Santos, Joao H. P. M. ^{[2, 1]} ; Bharmoria, Pankaj ^[1] ; Acosta, Mario A. Torres ^[3] ; Dias, V, Ana C. R. ; Coutinho, Joao A. P. ^[1] ; Ventura, Sonia P. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Aveiro, CICECO Aveiro Inst Mat, Dept Chem, Campus Univ Santiago, P-3810193 Aveiro - Portugal

^[2] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil

^[3] UCL, Dept Biochem Engn, Adv Ctr Biochem Engn, London WC1E 6BT - England

• Tipo de documento: Artigo Científico
• Fonte: ACS SUSTAINABLE CHEMISTRY & ENGINEERING; v. 9, n. 10, p. 3942-3954, MAR 15 2021.
• Citações Web of Science: 0

Resumo

Phycobiliproteins are fluorescent proteins mainly produced by red macroalgae and cyanobacteria. These proteins, essential to the survival of these organisms, find application in many fields of interest, from medical, pharmaceutical, and cosmetic to food and textile industries. The biggest obstacle to their use is the lack of simple environmental and economical sustainable methodologies to 1 obtain these proteins with high purity. In this work, a new purification process is proposed based on the induced precipitation of the target proteins followed by ultrafiltration. Purities of 89.5% of both phycobiliproteins and 87.3% of R-phycoerythrin were achieved using ammonium sulfate and poly(acrylic acid) sodium salts as precipitation agents (followed by an ultrafiltration step), while maintaining high recovery yields and protein structure stability. Environmental analysis performed to evaluate the proposed process shows that the carbon footprint for the proposed process is much lower than that reported for alternative methodology, and the economic analysis reveals the cost-effective character associated to its high performance. This work is a step toward more sustainable and effective methodologies/processes with high industrial potential. (AU)

• Processo FAPESP: 18/25994-2 - Desenvolvimento de novas plataformas de PEGuilação de proteínas com potencial terapêutico com recurso à microfluídica
• Beneficiário: João Henrique Picado Madalena Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado