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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sustainable Strategy Based on Induced Precipitation for the Purification of Phycobiliproteins

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Author(s):
Martins, Margarida [1] ; Soares, Bruna P. [1] ; Santos, Joao H. P. M. [2, 1] ; Bharmoria, Pankaj [1] ; Acosta, Mario A. Torres [3] ; Dias, V, Ana C. R. ; Coutinho, Joao A. P. [1] ; Ventura, Sonia P. M. [1]
Total Authors: 8
Affiliation:
[1] Univ Aveiro, CICECO Aveiro Inst Mat, Dept Chem, Campus Univ Santiago, P-3810193 Aveiro - Portugal
[2] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil
[3] UCL, Dept Biochem Engn, Adv Ctr Biochem Engn, London WC1E 6BT - England
Total Affiliations: 3
Document type: Journal article
Source: ACS SUSTAINABLE CHEMISTRY & ENGINEERING; v. 9, n. 10, p. 3942-3954, MAR 15 2021.
Web of Science Citations: 0
Abstract

Phycobiliproteins are fluorescent proteins mainly produced by red macroalgae and cyanobacteria. These proteins, essential to the survival of these organisms, find application in many fields of interest, from medical, pharmaceutical, and cosmetic to food and textile industries. The biggest obstacle to their use is the lack of simple environmental and economical sustainable methodologies to 1 obtain these proteins with high purity. In this work, a new purification process is proposed based on the induced precipitation of the target proteins followed by ultrafiltration. Purities of 89.5% of both phycobiliproteins and 87.3% of R-phycoerythrin were achieved using ammonium sulfate and poly(acrylic acid) sodium salts as precipitation agents (followed by an ultrafiltration step), while maintaining high recovery yields and protein structure stability. Environmental analysis performed to evaluate the proposed process shows that the carbon footprint for the proposed process is much lower than that reported for alternative methodology, and the economic analysis reveals the cost-effective character associated to its high performance. This work is a step toward more sustainable and effective methodologies/processes with high industrial potential. (AU)

FAPESP's process: 18/25994-2 - Development of novel platforms for PEGylation of proteins with therapeutic potential using microfluidics
Grantee:João Henrique Picado Madalena Santos
Support Opportunities: Scholarships in Brazil - Post-Doctoral