Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling

Autor(es)

Pereira Cipriano, Matheus Aparecido ^[1] ; Freitas-Iorio, Raquel De Paula ^[1] ; Dimitrov, Mauricio Rocha ^[2] ; Lopez de Andrade, Sara Adrian ^[3] ; Kuramae, Eiko Eurya ^{[2, 4]} ; Dias da Silveira, Adriana Parada ^[1]

Afiliação do(s) autor(es)

^[1] IAC, Ctr Solos & Recursos Agroambientais, Inst Agron, BR-13020902 Campinas, SP - Brazil

^[2] NIOO KNAW, Microbial Ecol Dept, Netherlands Inst Ecol, NL-6708 PB Wageningen - Netherlands

^[3] Univ Estadual Campinas, Dept Biol Vegetal, UNICAMP, BR-13083970 Campinas, SP - Brazil

^[4] Univ Utrecht, Inst Environm Biol, Ecol & Biodivers, NL-3584 CH Utrecht - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 9, n. 3 MAR 2021.
• Citações Web of Science: 1

Resumo

Beneficial plant-microbe interactions lead to physiological and biochemical changes that may result in plant-growth promotion. This study evaluated the effect of the interaction between sugarcane and endophytic bacterial strains on plant physiological and biochemical responses under two levels of nitrogen (N) fertilization. Six strains of endophytic bacteria, previously selected as plant growth-promoting bacteria (PGPB), were used to inoculate sugarcane mini stalks, with and without N fertilization. After 45 days, biomass production; shoot nutrient concentrations; foliar polyamine and free amino acid profiles; activities of nitrate reductase and glutamine synthase; and the relative transcript levels of the GS1, GS2, and SHR5 genes in sugarcane leaves were determined. All six endophytic strains promoted sugarcane growth, increasing shoot and root biomass, plant nutritional status, and the use efficiency of most nutrients. The inoculation-induced changes at the biochemical level altered the foliar free amino acid and polyamine profiles, mainly regarding the relative concentrations of citrulline, putrescine, glycine, alanine, glutamate, glutamine, proline, and aspartate. The transcription of GS1, GS2, and SHR5 was higher in the N fertilized seedlings, and almost not altered by endophytic bacterial strains. The endophytic strains promoted sugarcane seedlings growth mainly by improving nutrient efficiency. This improvement could not be explained by their ability to induce the production of amino acid and polyamine composts, or GS1, GS2, and SHR5, showing that complex interactions may be associated with enhancement of the sugarcane seedlings' performance by endophytic bacteria. The strains demonstrated biotechnological potential for sugarcane seedling production. (AU)

• Processo FAPESP: 08/56147-1 - Nutrição nitrogenada de cana-de-açúcar com fertilizantes ou bactérias diazotróficas
• Beneficiário: Heitor Cantarella
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático