Illuminating the Brain With X-Rays: Contributions and Future Perspectives of High-Resolution Microtomography to Neuroscience

Autor(es)

Rodrigues, Paulla Vieira ^{[1, 2]} ; Tostes, Katiane ^[1] ; Bosque, Beatriz Pelegrini ^{[1, 2]} ; Godoy, Joao Vitor Pereira de ^{[1, 2]} ; Amorim Neto, Dionisio Pedro ^{[1, 2]} ; Dias, Carlos Sato Baraldi ^[3] ; Fonseca, Matheus de Castro ^[1]

Afiliação do(s) autor(es)

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas - Brazil

^[2] Univ Estadual Campinas, Dept Struct & Funct Biol, Campinas - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Natl Lab LNLS, Campinas - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: FRONTIERS IN NEUROSCIENCE; v. 15, MAR 17 2021.
• Citações Web of Science: 0

Resumo

The assessment of three-dimensional (3D) brain cytoarchitecture at a cellular resolution remains a great challenge in the field of neuroscience and constant development of imaging techniques has become crucial, particularly when it comes to offering direct and clear obtention of data from macro to nano scales. Magnetic resonance imaging (MRI) and electron or optical microscopy, although valuable, still face some issues such as the lack of contrast and extensive sample preparation protocols. In this context, x-ray microtomography (mu CT) has become a promising non-destructive tool for imaging a broad range of samples, from dense materials to soft biological specimens. It is a new supplemental method to be explored for deciphering the cytoarchitecture and connectivity of the brain. This review aims to bring together published works using x-ray mu CT in neurobiology in order to discuss the achievements made so far and the future of this technique for neuroscience. (AU)

• Processo FAPESP: 19/24511-0 - LRRK2 e Rab GTPases na neurodegeneração causada pela Doença de Parkinson esporádica
• Beneficiário: Beatriz Pelegrini Bosque
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/20014-0 - Conectando o intestino e o cérebro: envolvimento do microbioma intestinal no surgimento e progressão da doença de Parkinson esporádica
• Beneficiário: Matheus de Castro Fonseca
• Modalidade de apoio: Auxílio à Pesquisa - Regular