Protein restriction during puberty alters nutritional parameters and affects ovarian and uterine histomorphometry in adulthood in rats

Autor(es)

de Morais Oliveira, Diego Augusto ^{[1, 2]} ; Lupi, Luiz Antonio ^[2] ; Silveira, Henrique Spaulonci ^[2] ; de Almeida Chuffa, Luiz Gustavo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biosci Botucatu, Grad Program Nutr, Sao Paulo - Brazil

^[2] UNESP Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, POB 18618-689, Rubiao Jr S-N, BR-510 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Experimental Pathology; MAR 2021.
• Citações Web of Science: 0

Resumo

In a large part of the population inefficient ingestion of proteins, whether for cultural, aesthetic or economic reasons, is a global concern. Low-protein diets can cause severe functional complications, mainly during the development and maturation of organs and systems, including the female reproductive system. The present study investigated the effect of nutritional protein restriction during puberty on the oestrous cycle and expression of sex steroid receptors (AR, ER alpha e ER beta) in ovarian and uterine tissues of adult rats. Protein restriction promoted lower body weight gain, feed efficiency and higher caloric intake. There was an increase in the oestrus phase arrest without changing the total length of the oestrous cycle. The consumption of low-protein diet also reduced the thickness of the uterine endometrium (uterine epithelium and endometrial stroma) in addition to increasing the number of primary and atretic follicles in the ovaries. Furthermore, the low-protein diet reduced the levels of androgen receptor (AR) and increased the oestrogen receptor beta (ER beta) in the ovary, while no significant changes were observed in the uterus. Our study reinforces the importance of adequate protein intake during puberty, since physiological changes in this developmental period interfere with the histomorphometry of the ovaries and uteri, possibly resulting in impaired folliculogenesis and fertility in the reproductive period. (AU)

• Processo FAPESP: 19/00906-6 - Melatonina e seus receptores MT1 e MT2: efeitos na apoptose, proliferação e potencial migratório de células de carcinoma ovariano (linhagem SKOV-3)
• Beneficiário: Luiz Gustavo de Almeida Chuffa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/09243-7 - Impacto da restrição proteica sobre o ciclo estral e receptores esteróides sexuais no ovário e útero de ratas juvenis
• Beneficiário: Diego Augusto de Morais Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica