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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Experimental dentin adhesives containing calcium orthophosphate particles: Effect on dentin bond strength, micro-permeability and collagen degradation

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Autor(es):
Chiari, Marina D. S. [1] ; Alania, Yvette [2] ; Bedran-Russo, Ana K. [2] ; Braga, Roberto R. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Biomat & Oral Biol, Sch Dent, Av Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil
[2] Marquette Univ, Dept Gen Dent Sci, Milwaukee, WI 53233 - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES; v. 107, JUN 2021.
Citações Web of Science: 0
Resumo

Purpose: To assess the effect of dicalcium phosphate dihydrate (DCPD) or mineral trioxide aggregate (MTA) particles on the microtensile bond strength (mu TBS), micro-permeability and enzymatic activity at the adhesive-dentin interface of experimental dental adhesives. Materials and methods: An experimental ethanol-based, two-step etch-and-rinse adhesive system was prepared. Four experimental groups were tested, based on the type of particle added: (1) DCPD functionalized with diethylene glycol dimethacrylate (DCPD\_DEGDMA), (2) citric acid (DCPD\_CA), (3) calcium silicate (MTA) and (4) unfilled (control). All particles were added at 10 wt%. Human third molars had their occlusal enamel removed and dentin surface polished. After acid etching, the adhesives were applied on the dentin surfaces and the teeth were restored with a commercial nanofilled resin composite. The mu TBS and micro-permeability were tested after 24 h or 2 months of storage in simulated body fluid (SBF) at 37 degrees C. Bonded interfaces were observed using scanning electron microcopy (SEM). Gelatinolytic activity at the adhesive interfaces was assessed by in situ zymography at 24 h and 2 months in SBF/37 degrees C. The results were described as relative florescent units (RFU). mu TBS data were analyzed by ANOVA/Games-Howell test. Data from micro-permeability and in situ zymography were analyzed by Kruskal-Wallis/Mann-Whitney test (alpha: 5%). Results: Differences in mu TBS were found among adhesives (control = MTA > DCPD\_CA > DCPD\_DEGDMA, p < 0.001), but not between storage times. The control group showed the lowest permeability at both observation periods. Except for DCPD\_CA, no significant increases in micro-permeability were observed after 2 months. All groups presented significant reductions in RFU values between 24 h and 2 months. DCPD\_DEGDMA (at 24 h) and MTA (at 2 months) presented statistically lower RFU than the control (p < 0.01). Conclusion: Evidence of reduced MMP activity was observed at adhesive interfaces with the use of experimental adhesives containing calcium-releasing particles. However, after two months, bond strength and micro-permeability results suggest that interfacial stability was not affected by adhesive formulation. (AU)

Processo FAPESP: 16/25971-7 - Desenvolvimento e aplicação de nanopartículas de fosfato de cálcio funcionalizadas com monômeros ácidos para remineralização dentária
Beneficiário:Marina Damasceno e Souza de Carvalho Chiari
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 18/02403-9 - Efeito sobre a resistência de união e degradação de colágeno de adesivos dentinários experimentais contendo partículas de ortofosfato de cálcio
Beneficiário:Marina Damasceno e Souza de Carvalho Chiari
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado