Detection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species

Autor(es)

Cornacini, Maiara R. ^[1] ; Manoel, Ricardo O. ^[2] ; Alcantara, Marcelo A. M. ^[1] ; Moraes, Mario L. T. ^[3] ; Silva, Edvaldo A. A. ^[4] ; Pereira Neto, Leonel G. ^[5] ; Sebbenn, Alexandre M. ^[6] ; Rossini, Bruno C. ^{[1, 2]} ; Marino, Celso L. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Biociencias, Dept Ciencias Quim & Biol, Botucatu, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Inst Biotecnol IBTEC, Botucatu, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Fitotecnia Tecnol Alimentos & Socio Econ, Ilha Solteira - Brazil

^[4] UNESP Univ Estadual Paulista, Dept Prod Vegetal, Fac Ciencias Agron, Botucatu, SP - Brazil

^[5] Embrapa Recursos Genet & Biotecnol Cenargen, Brasilia, DF - Brazil

^[6] Inst Florestal Sao Paulo, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 48, n. 4 APR 2021.
• Citações Web of Science: 0

Resumo

Astronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (G(ST) = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. (AU)

• Processo FAPESP: 18/00898-0 - Diversidade genética, fluxo gênico e estrutura espacial de genótipos de Gonçalo-alves (Astronium fraxinifolium) em área degradada
• Beneficiário: Ricardo de Oliveira Manoel
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado