Antimicrobial and anthelmintic activities of the ethanolic extract, fractions and isolated compounds from Manilkara zapota L. P. Royen (Sapotaceae)

Autor(es)

Mourao Mulvaney, Layany Carolyny ^{[1, 2]} ; Xavier-Junior, Francisco Humberto ^[3] ; Rodrigues, Alice M. S. ^[4] ; Stien, Didier ^{[2, 4]} ; Allegretti, Silmara Marques ^[5] ; Garcia, Vera Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Chem Biol & Agr Pluridisciplinary Res Ctr, Campinas, SP - Brazil

^[2] CNRS, Inst Chim Subst Nat, Gif Sur Yvette - France

^[3] Univ Potiguar UnP, Programa Posgrad Biotecnol, Campus Salgado Filho, Natal, RN - Brazil

^[4] Sorbonne Univ, UPMC Univ Paris 06, CNRS, Observ Oceanol, Lab Biodiversite & Biotechnol Microbiennes, LBBM, Banyuls Sur Mer - France

^[5] Univ Estadual Campinas, Inst Biol, Dept Anim Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmacy and Pharmacology; v. 73, n. 3, p. 377-387, MAR 6 2021.
• Citações Web of Science: 0

Resumo

Objectives To evaluate the antibacterial, antifungal and anthelmintic activities of the ethanolic extract (EEMz), fractions (LPFMz and HPFMz) and compounds isolated from the leaves of Manilkara zapota L. P. Royen. Methods Extract and fractions were produced by turbolization. LPFMz fraction was analysed by gas chromatography-mass spectrometry. The isolated compounds from HPFMz were purified by flash and preparative chromatographic methods, and chemically characterised by UPLC-ESITOFMS, optical rotation, and one- and two-dimensional H-1 and C-13 NMR techniques. Anthelmintic against Strongyloides venezuelensis and antimicrobial activities against Candida albicans, Trichophyton rubrum and Staphylococcus aureus were evaluated. Key findings EEMz showed mainly phenolic compounds and pentacyclic triterpenes from Delta 12-oleane/Delta 12-ursane series. Chlorogenic acid, myricetin-3-O-beta -D-glucopyranoside, mearnsitrin, germanicol and germanicol acetate were reported to M. zapota leaves for the first time in this work. EEMz, HPFMz, LPFMz showed significative activity against C. albicans (16 mu g/mL), while isolated flavonoids were active against S. aureus (<32 <mu>g/mL). EEMz, phenolic-rich compounds (F-2), and chlorogenic acid were potentially active against S. venezuelensis at 24 h. Conclusions M. zapota and its bioactive compound can be eligible such as a potential phytomedicine for the treatment of microbial and strongyloidiasis drug-resistant infections. (AU)

• Processo FAPESP: 13/05355-1 - Estudo fitoquímico biomonitorado de extratos de Manilkara zapota L. e Labramia bojeri A. com atividade frente ao Strongyloides venezuelensis demonstrada por ensaios in vitro
• Beneficiário: Vera Lúcia Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular