Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil

Autor(es)

Lima, Ana Carolina S. ^{[1, 2]} ; Gomes, Claudia Maria C. ^[1] ; Tomokane, Thaise Y. ^[1] ; Campos, Marliane Batista ^[2] ; Zampieri, Ricardo A. ^[3] ; Jorge, Carolina L. ^[3] ; Laurenti, Marcia D. ^[1] ; Silveira, Fernando T. ^[2] ; Corbett, Carlos Eduardo P. ^[1] ; Floeter-Winter, Lucile Maria ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept DePatol, Sao Paulo, SP - Brazil

^[2] Inst Evandro Chagas, Secretaria Vigilancia Saude, Minist Saude, Belem, Para - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 44, n. 2 2021.
• Citações Web of Science: 0

Resumo

Abstract Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular targets were employed to check the hybrid identity of the putative hybrids. Heat shock protein 70 (hsp70) gene sequences were analyzed by three different polymerase chain reaction (PCR) approaches, and two different patterns of inherited hsp70 alleles were found. Three isolates presented heterozygous L. (V.) guyanensis/L. (V.) shawi patterns, and four presented homozygous hsp70 patterns involving only L. (V.) shawi alleles. The amplicon sequences confirmed the RFLP patterns. The high-resolution melting method detected variant heterozygous and homozygous profiles. Single-nucleotide polymorphism genotyping/cleaved amplified polymorphic site analysis suggested a higher contribution from L. (V.) guyanensis in hsp70 heterozygous hybrids. Additionally, PCR-RFLP analysis targeting the enzyme mannose phosphate isomerase (mpi) gene indicated heterozygous and homozygous cleavage patterns for L. (V.) shawi and L. (V.) guyanensis, corroborating the hsp70 findings. In this communication, we present molecular findings based on partial informative regions of the coding sequences of hsp70 and mpi as markers confirming that some of the parasite strains from the Brazilian Amazon region are indeed hybrids between L. (V.) guyanensis and L. (V.) shawi. (AU)

• Processo FAPESP: 14/50315-0 - Leishmanioses na América Latina: uma perspectiva avançada sobre fatores imunopatogenéticos da infecção cutânea e visceral, imunomoduladores da saliva de vetores flebotomíneos e exo-antígenos imunogênicos de Leishmania (L.) infantum chagasi candidatos à vacina
• Beneficiário: Marcia Dalastra Laurenti
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/08338-8 - Caracterização Biológica e Molecular de cepas de Leishmania sp. isoladas de pacientes com leishmaniose tegumentar americana oriundos da região amazônica, Santarém-PA, Brasil.
• Beneficiário: Ana Carolina Stocco de Lima
• Modalidade de apoio: Bolsas no Brasil - Doutorado