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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Subgingival endotoxin and lipoteichoic acid modulate cytokine production in diabetic subjects: A Case-control Study

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Autor(es):
Rangel, Thiago P. [1] ; Reis, Aurelio A. [1] ; Caponi, Lara [1] ; Pena, Larissa C. S. [1] ; Ruiz, Karina G. S. [1] ; Santamaria, Mauro P. [2] ; Mathias-Santamaria, Ingrid F. [2] ; Casati, Marcio Z. [1] ; Casarin, Renato C. V. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dent Sch Piracicaba, Dept Prosthodont & Periodont, Campinas - Brazil
[2] Sao Paulo State Univ, Div Periodontol, Coll Dent, FOSJC, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ORAL DISEASES; v. 27, n. 5, p. 1325-1333, JUL 2021.
Citações Web of Science: 1
Resumo

Background Periodontal disease and diabetes mellitus (DM) are highly prevalent and interrelated diseases, resulting in altered host response microbiota. Thus, this study aimed to evaluate the impact of DM on local levels of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and their relationship with cytokines and matrix metalloproteinases' (MMPs) profile. Methods This case-control study included diabetic (n = 15) and non-diabetic (n = 15) subjects presenting Stage 3-4, Grade C, Periodontitis. Gingival crevicular fluid (GCF) was collected, and LPS and LTA levels were analyzed by enzyme-linked immunosorbent assay (ELISA), while IFN-gamma, IL-10, IL-17, IL-1 beta, IL-4, MMP-2, and MMP-9 were measured by LUMINEX/MAGpix. Mann-Whitney and Spearman's correlation tests were used to compared and to correlate variables (p < 0.05). Results Higher levels of LTA, LPS, IL-10, IL-1 beta, and MMP-2 (p < 0.05) and lower levels of IL-17 were found in the DM group (p < 0.05). Non-diabetic subjects presented higher LPS, IFN-gamma, IL-17, and MMP-2 levels and lower IL-10 concentration (p < 0.05). No significant correlation was seen between LPS and cytokine profile in non-diabetic. Local levels of LTA were positively correlated with IL-17 and MMP-2 and negatively with IL-10. Conclusion LTA and LPS drove the inflammatory profile through the modulation of cytokines and MMPs in a different manner in DM and non-diabetic subjects. (AU)

Processo FAPESP: 18/12208-9 - Relação entre os níveis de ácido lipoteicóico e perfil de citocinas no ambiente subgengival de indivíduos diabéticos: estudo de caso controle
Beneficiário:Larissa Cristina Spirito Pena
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica