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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Subgingival endotoxin and lipoteichoic acid modulate cytokine production in diabetic subjects: A Case-control Study

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Author(s):
Rangel, Thiago P. [1] ; Reis, Aurelio A. [1] ; Caponi, Lara [1] ; Pena, Larissa C. S. [1] ; Ruiz, Karina G. S. [1] ; Santamaria, Mauro P. [2] ; Mathias-Santamaria, Ingrid F. [2] ; Casati, Marcio Z. [1] ; Casarin, Renato C. V. [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Campinas, Dent Sch Piracicaba, Dept Prosthodont & Periodont, Campinas - Brazil
[2] Sao Paulo State Univ, Div Periodontol, Coll Dent, FOSJC, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ORAL DISEASES; v. 27, n. 5, p. 1325-1333, JUL 2021.
Web of Science Citations: 1
Abstract

Background Periodontal disease and diabetes mellitus (DM) are highly prevalent and interrelated diseases, resulting in altered host response microbiota. Thus, this study aimed to evaluate the impact of DM on local levels of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and their relationship with cytokines and matrix metalloproteinases' (MMPs) profile. Methods This case-control study included diabetic (n = 15) and non-diabetic (n = 15) subjects presenting Stage 3-4, Grade C, Periodontitis. Gingival crevicular fluid (GCF) was collected, and LPS and LTA levels were analyzed by enzyme-linked immunosorbent assay (ELISA), while IFN-gamma, IL-10, IL-17, IL-1 beta, IL-4, MMP-2, and MMP-9 were measured by LUMINEX/MAGpix. Mann-Whitney and Spearman's correlation tests were used to compared and to correlate variables (p < 0.05). Results Higher levels of LTA, LPS, IL-10, IL-1 beta, and MMP-2 (p < 0.05) and lower levels of IL-17 were found in the DM group (p < 0.05). Non-diabetic subjects presented higher LPS, IFN-gamma, IL-17, and MMP-2 levels and lower IL-10 concentration (p < 0.05). No significant correlation was seen between LPS and cytokine profile in non-diabetic. Local levels of LTA were positively correlated with IL-17 and MMP-2 and negatively with IL-10. Conclusion LTA and LPS drove the inflammatory profile through the modulation of cytokines and MMPs in a different manner in DM and non-diabetic subjects. (AU)

FAPESP's process: 18/12208-9 - Relationship between cytokine pattern and lipoteichoic acid in the subgingival environment of diabetics and non-diabetics subjects: a case-control study
Grantee:Larissa Cristina Spirito Pena
Support Opportunities: Scholarships in Brazil - Scientific Initiation