INFLUENCE OF THE INFECTION TIME ON THE MICROBIOLOGICAL (BACTERIA AND FUNGI), CYTOTOXIC AND INFLAMMATORY PROFILE OF THE MICROBIAL COMMUNITY OF SECONDARY/PERSISTENT ENDODONTIC INFECTIONS

• Grant number: 21/14570-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2022
• End date: May 31, 2025
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Brenda Paula Figueiredo de Almeida Gomes
• Grantee: Ederaldo Pietrafesa de Godoi Junior
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated research grant: 15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections, AP.TEM

Abstract

The permanence of microorganisms in the root canal system (SCR) is the main etiological factor for the failure of endodontic treatment. The root canal microbiota, its cytotoxic profile and the host's immune system are the main responsible for the course of apical inflammation, as well as for the success and longevity of endodontic treatment. Considering the importance of persistent microorganisms to endodontic treatment, their antigenic activity and interaction with the host organism for the success of endodontic treatment, this study aims to characterize and to evaluate the microbiota (bacteria/fungi) of cases indicated for conventional endodontic retreatment due to the presence of chronic apical periodontitis after different times of clinical failure, as well as the levels of endotoxins (LPS) and lipoteichoic acid (LTA) associated with endodontic infections. For identification and detection of microorganisms will be selected 40 patients in need of endodontic retreatment due to the presence of periapical lesion subdivided into 4 groups determined by the time elapsed since the primary endodontic treatment. The microbiological, cytotoxic and cytokine content will be collected from the SCR using the sterile absorbent paper cone technique. The extracted DNA will be submitted to NGS (Next Generation Sequencing) sequencing. Endotoxin (LPS) concentrations will be determined using the LAL turbidimetric method. There will also be an immunoenzymatic ELISA assay for quantification of lipoteichoic acid (LTA). The data obtained will be analyzed through bioinformatics pipelines for diversity, while the results of the other studies will be properly tabulated and statistically analyzed using SPSS 23 for Windows.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GODOI-JR, EDERALDO P.; BRONZATO, JULIANA D.; FRANCISCO, PRISCILA A.; BICEGO-PEREIRA, ELOA C.; LOPES, ERICA M.; PASSINI, MAICON R. Z.; DE-JESUS-SOARES, ADRIANA; ALMEIDA, JOSE F. A.; MARCIANO, MARINA A.; FERRAZ, CAIO C. R.; et al. Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons. CLINICAL ORAL INVESTIGATIONS, v. 27, n. 5, p. 16-pg., 2023-03-31. (19/14448-0, 17/16516-7, 17/25090-3, 21/13871-6, 21/14570-0)

CHIARELLI-NETO, VITO M.; DE AVEIRO, EMELLY; BRONZATO, JULIANA D.; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; GODOI, EDERALDO P.; LOPES, ERICA M.; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO C. R.; ALMEIDA, JOSE F. A.; et al. Clinical evaluation of culturable bacteria, endotoxins and lipoteichoic acid in teeth with vital normal pulp tissues. AUSTRALIAN ENDODONTIC JOURNAL, v. N/A, p. 9-pg., 2023-08-01. (21/13871-6, 15/23479-5, 19/14448-0, 21/14570-0, 17/25242-8, 17/25090-3, 19/19300-0)