Beyond host specificity: the biotechnological exploitation of chitolectin from teratocytes ofToxoneuron nigricepsto control non-permissive hosts

Autor(es)

Merlin, Bruna Lais ^[1] ; Pino, Lilian Ellen ^{[2, 3]} ; Peres, Lazaro Eustaquio Pereira ^[3] ; Prataviera, Fabio ^[4] ; Ortega, Edwin Moises Marcos ^[4] ; Consoli, Fernando Luis ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Entomol & Acarol, Insect Interact Lab, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Ctr Nucl Energy Agr, Plant Breeding Lab, Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, Dept Biol Sci, Luiz de Queiroz Coll Agr, Hormonal Control Plant Dev Lab, Piracicaba, SP - Brazil

^[4] Univ Sao Paulo, Dept Exact Sci, Luiz de Queiroz Coll Agr, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PEST SCIENCE; v. 94, n. 3, p. 713-727, JUN 2021.
• Citações Web of Science: 0

Resumo

The virulence factors (VF) that parasitoids use to regulate the growth and development of their hosts have potential as new events in transgenic plants. VFs are promising candidates for biotechnological exploitation and implementation of pest-control tools to meet the world's demand for food in 2100, while sustainably increasing agricultural production. However, VFs have not been used in commercial transgenic plants, since they are thought to target only a narrow range of insects, due to the parasitoid host specialization. We investigated the potential of chitolectin (TnChit), produced by the teratocytes ofToxoneuron nigriceps(Viereck) (Hymenoptera: Braconidae), as a transgenic event to control several non-permissive hosts:Chrysodeixis includens(Walker),Spodoptera albula(Walker),Spodoptera frugiperda(JE Smith) (Lepidoptera: Noctuidae),Tuta absoluta(Meyrick) (Lepidoptera: Gelechiidae) andBemisia tabaci(Gennadius) (Hemiptera: Aleyrodidae). Third instars ofC. includens,S. albulaandS. frugiperdahad lower survival and probability of molting onTnChit-modified plants than on control plants. The nutritional indices ofS. frugiperdawere also altered. Larval survival and pupal weight of males ofT. absolutawere reduced on transgenic plants. Adult emergence ofB. tabaciwas also reduced. The expression of theTnChitgene differed in the transformation events obtained and was consistent with the biological effects observed. The effects ofTnChiton non-permissive hosts demonstrate the potential of the virulence factors that parasitoids employ in host regulation, as a source of new genes in plant transformation for control of insect pests. (AU)

• Processo FAPESP: 05/00355-7 - Interações hospedeiro-parasitóide no desenvolvimento de técnicas para o controle biológico de pragas
• Beneficiário: Fernando Luis Cônsoli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/16553-1 - Ação da citocinina e sua interação com outros hormônios vegetais no controle da ramificação em tomateiro (Solanum lycopersicum L.)
• Beneficiário: Lilian Ellen Pino
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado