Osmotic and ionic regulation, and kinetic characte... - BV FAPESP
Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Osmotic and ionic regulation, and kinetic characteristics of a posterior gill (Na+, K+)-ATPase from the blue crab Callinectes danae on acclimation to salinity challenge

Texto completo
Autor(es):
Garcon, Daniela P. [1] ; Leone, Francisco A. [2] ; Faleiros, Rogerio O. [3] ; Pinto, Marcelo R. [4] ; Moraes, Cintya M. [5] ; Fabri, Leonardo M. [5] ; Antunes, Claudia D. [6] ; McNamara, John Campbell [7, 6]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Triangulo Mineiro, Campus Univ Iturama, Iturama, MG - Brazil
[2] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Univ Fed Espirito Santo, Dept Ciencias Agr & Biol, Sao Mateus, ES - Brazil
[4] Univ Uberaba, Lab Biopatol & Biol Mol, Uberaba, MG - Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[7] Univ Sao Paulo, Ctr Biol Marinha, Sao Sebastiao, SP - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: Marine Biology; v. 168, n. 6 JUN 2021.
Citações Web of Science: 0
Resumo

Salt tolerance reflects ecophysiological adaptation, and the wide-ranging distribution of the Brachyura mirrors their ability to adjust body fluid concentrations. The gill (Na+, K+)-ATPase underpins such hyper/hypo-regulatory mechanisms. We evaluate osmotic and chloride regulation in Callinectes danae after 10 days acclimation to a wide salinity range (5-50 parts per thousand S), accompanying alterations in hemolymph osmolality and {[}Cl-] during hypo- (15 parts per thousand S) or hyper- (45 parts per thousand S) osmotic challenge. Further, we investigate posterior gill (Na+, K+)-ATPase kinetics, alpha-subunit immunolocalization and its mRNA and protein expression (15, 30 and 40 or 45 parts per thousand S). The crab is a moderate, asymmetrical hyper/hypo-osmoregulator but is a strong, asymmetrical hyper/hypo-chloride regulator. Hyper-regulation at low salinity is sustained by a threefold increase in (Na+, K+)-ATPase activity, a 3.5-fold increase in alpha-subunit mRNA expression and 1.6-fold increase in protein expression. alpha-Subunit signal is highest in 15 parts per thousand S-acclimated crabs, and is uniformly distributed throughout the ionocytes and pillar cells. Activity in 30- and 40% S-acclimated crabs is similar. Affinity for ATP and Na+ increases on high salinity acclimation but decreases for ouabain. K+ apparent affinity is independent of salinity, while that for Mg2+ decreases and for NH4+ increases with increasing salinity. A high-affinity ATP-binding site disappears on acclimation at any salinity. FOF1- and Na+- or K+-ATPase activities decrease with increased salinity. Hemolymph chloride hypo-regulation depends little on gill (Na+, K+)-ATPase activity. Hyper-, and hypo-osmotic and ionic regulatory capabilities in C. danae are intricate physiological processes underpinned by multifarious gill (Na+, K+)-ATPase kinetics and altered mRNA and protein expressions. (AU)

Processo FAPESP: 15/00131-3 - A secreção de cloreto pelo epitélio branquial em camarões e caranguejos de diferentes nichos osmóticos: uma investigação fisiológica e molecular do simportador Na+-K+-2Cl-
Beneficiário:John Campbell McNamara
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/04870-9 - Estratégias fisiológicas adaptativas nos crustáceos: a invasão da água doce e as bases moleculares e estruturais da osmorregulação
Beneficiário:John Campbell McNamara
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/50216-9 - A base ultraestrutural da regulacao osmotica e ionica no siri azul, callinectes danae (crustacea, brachyura).
Beneficiário:Cláudia Doi Antunes
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 16/25336-0 - Estudo bioquímico comparativo da (Na+, K+)-ATPase branquial de Macrobrachium amazonicum (Heller, 1862) habitantes de regiões costeiras e continentais
Beneficiário:Francisco de Assis Leone
Modalidade de apoio: Auxílio à Pesquisa - Regular