Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares

Autor(es)

Segabinazzi, Lorenzo G. T. M. ^{[1, 2, 3]} ; Canisso, Igor F. ^[2] ; Podico, Giorgia ^[2] ; Cunha, Lais L. ^[2] ; Novello, Guilherme ^{[1, 2]} ; Rosser, Michael F. ^[4] ; Loux, Shavahn C. ^[5] ; Lima, Fabio S. ^{[6, 2]} ; Alvarenga, Marco A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anim Reprod, BR-18618681 Botucatu, SP - Brazil

^[2] Univ Illinois, Coll Vet Med, Dept Vet Clin Med, 1008 W Hazelwood Dr, Urbana, IL 61802 - USA

^[3] Ross Univ, Sch Vet Med, POB 334, Basseterre, West Indies - St Kitts & Nevi

^[4] Univ Illinois, Coll Vet Med, Vet Diagnost Lab, Urbana, IL 61802 - USA

^[5] Univ Kentucky, Maxwell H Gluck Equine Res Ctr, Lexington, KY 40503 - USA

^[6] Univ Calif Davis, Sch Vet Med, Dept Populat Hlth & Reprod, Davis, CA 95616 - USA

• Tipo de documento: Artigo Científico
• Fonte: ANTIBIOTICS-BASEL; v. 10, n. 5 MAY 2021.
• Citações Web of Science: 1

Resumo

Microorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or -poor (PPP) plasma. Mares (n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre- (-48 and -24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Il beta, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey's post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property. (AU)

• Processo FAPESP: 18/02856-3 - Aspectos clínicos, moleculares e proteômica do útero de éguas com endometrite tratadas com terapias biológicas: plasma rico em plaquetas e meio condicionado por células tronco; e firocoxib.
• Beneficiário: Marco Antonio Alvarenga
• Modalidade de apoio: Auxílio à Pesquisa - Regular