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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares

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Author(s):
Segabinazzi, Lorenzo G. T. M. [1, 2, 3] ; Canisso, Igor F. [2] ; Podico, Giorgia [2] ; Cunha, Lais L. [2] ; Novello, Guilherme [1, 2] ; Rosser, Michael F. [4] ; Loux, Shavahn C. [5] ; Lima, Fabio S. [6, 2] ; Alvarenga, Marco A. [1]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anim Reprod, BR-18618681 Botucatu, SP - Brazil
[2] Univ Illinois, Coll Vet Med, Dept Vet Clin Med, 1008 W Hazelwood Dr, Urbana, IL 61802 - USA
[3] Ross Univ, Sch Vet Med, POB 334, Basseterre, West Indies - St Kitts & Nevi
[4] Univ Illinois, Coll Vet Med, Vet Diagnost Lab, Urbana, IL 61802 - USA
[5] Univ Kentucky, Maxwell H Gluck Equine Res Ctr, Lexington, KY 40503 - USA
[6] Univ Calif Davis, Sch Vet Med, Dept Populat Hlth & Reprod, Davis, CA 95616 - USA
Total Affiliations: 6
Document type: Journal article
Source: ANTIBIOTICS-BASEL; v. 10, n. 5 MAY 2021.
Web of Science Citations: 1
Abstract

Microorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or -poor (PPP) plasma. Mares (n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre- (-48 and -24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Il beta, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey's post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property. (AU)

FAPESP's process: 18/02856-3 - Clinical, molecular and proteomic aspects of the uterus of susceptible mares treated with biological therapies: platelet rich plasma and stem cells conditioned medium; and firocoxib.
Grantee:Marco Antonio Alvarenga
Support Opportunities: Regular Research Grants