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Differential gene expression among persistent post-breeding endometritis resistant and susceptible mares

Grant number: 14/11117-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): December 01, 2014
Effective date (End): November 30, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:João Pessoa Araújo Junior
Grantee:Eduardo Gorzoni Fioratti
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

After mating, the mares' uterus has shown a physiological inflammatory response induced by semen components, especially spermatozoa, which is responsible for cleaning the uterine contents. Besides, some mares exhibit this inflammation as persistent way resulting in decline on fertility. The aim of this study will be to verify differences in gene expression of persistent post-breeding endometritis resistant and susceptible mares during hormonal change occurred between estrous and diestrous and to characterize through gene expression and metabolic pathways of the uterine inflammation after artificial insemination. Were pre-selected 10 resistant and 10 susceptible mares to persistent post- breeding endometritis according to decrease on fertility, uterine cytology, structural anatomic changes and intrauterine fluid accumulation after the mating. Two consecutive estrous cycles were monitored and randomly selected as control (without IA) or treated (IA with 1x109 total sperm) groups. AIs were performed 24 h after ovulation induction, which occurred between 24 and 48 h after the induction. Uterine exfoliative cytology were performed 24 (M1), 48 (M2) and 72h (M3) after the ovulation induction and the endometrial cells were stored in RNAlater ® at -20 °C. Total RNA was extracted from the samples, treated with DNAse and the cDNA was obtained to prepare the library for transcriptome analysis (RNAseq), the differentially expressed genes (up and down-regulated) and may play important roles will be chosen to be confirmed and quantified by qPCR. (AU)