Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism

Autor(es)

Kuechler, Erika Calvano ^[1] ; Reis, Caio Luiz Bitencourt ^[2] ; Maranon-Vasquez, Guido ^[3] ; Nelson-Filho, Paulo ^[2] ; Matsumoto, Mirian Aiko Nakane ^[2] ; Stuani, Maria Bernadete Sasso ^[2] ; Oliveira, Maria Angelica Hueb de Menezes ^[4] ; Proff, Peter ^[1] ; Kirschneck, Christian ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Regensburg, Dept Orthodont, Franz Josef Strauss Allee 11, D-93053 Regensburg - Germany

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Av Cafe S-N, BR-14040904 Ribeirao Preto - Brazil

^[3] Univ Fed Rio de Janeiro, Sch Dent, Dept Pediat Dent & Orthodont, R Prof Rodolpho Paulo Rocco 325, BR-21941617 Rio De Janeiro - Brazil

^[4] Univ Uberaba, Sch Dent, R Frei Paulino 30, BR-38025180 Uberaba, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PERSONALIZED MEDICINE; v. 11, n. 5 MAY 2021.
• Citações Web of Science: 0

Resumo

In this study we evaluated whether single nucleotide polymorphisms (SNPs) in the genes encoding PTH, VDR, CYP24A1, and CYP27B1 were associated with mandibular retrognathism (MR). Samples from biologically-unrelated Brazilian patients receiving orthodontic treatment were included in this study. Pre-orthodontic lateral cephalograms were used to determine the phenotype. Patients with a retrognathic mandible were selected as cases and those with an orthognathic mandible were selected as controls. Genomic DNA was used for genotyping analysis of SNPs in PTH (rs694, rs6256, and rs307247), VDR (rs7975232), CYP24A1 (rs464653), and CYP27B1 (rs927650). Chi-squared or Fisher's tests were used to compare genotype and allele distribution among groups. Haplotype analysis was performed for the SNPs in PTH. The established alpha was p < 0.05. Multifactor dimensionality reduction (MDR) was used to identify SNP-SNP interactions. A total of 48 (22 males and 26 females) MR and 43 (17 males and 26 females) controls were included. The linear mandibular and the angular measurements were statistically different between MR and controls (p < 0.05). In the genotype and allele distribution analysis, the SNPs rs694, rs307247, and rs464653 were associated with MR (p < 0.05). MDR analyses predicted the best interaction model for MR was rs694-rs927650, followed by rs307247-rs464653-rs927650. Some haplotypes in the PTH gene presented statistical significance. Our results suggest that SNPs in PTH, VDR, CYP24A1, and CYP27B1 genes are associated with the presence of mandibular retrognathism. (AU)

• Processo FAPESP: 15/06866-5 - Avaliação do papel do estrógeno no desenvolvimento dentofacial
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores