| Texto completo | |
| Autor(es): |
Garbieri, Thais Francini
[1, 2]
;
Martin, Victor
[2, 3]
;
Santos, Carlos Ferreira
[1]
;
Gomes, Pedro de Sousa
[2, 3]
;
Fernandes, Maria Helena
[2, 3]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil
[2] Univ Porto, Fac Med Dent, Lab Bone Metab & Regenerat, P-4200393 Porto - Portugal
[3] Univ Porto, LAQV REQUIMTE, P-4160007 Porto - Portugal
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PHARMACEUTICALS; v. 14, n. 5 MAY 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Activation of renin-angiotensin system (RAS) plays a role in bone deterioration associated with bone metabolic disorders, via increased Angiotensin II (AngII) targeting Angiotensin II type 1 receptor/Angiotensin II type 2 receptor (AT1R/AT2R). Despite the wide data availability, the RAS role remains controversial. This study analyzes the feasibility of using the embryonic chick femur organotypic model to address AngII/AT1R/AT2R axis in bone, which is an application not yet considered. Embryonic day-11 femurs were cultured ex vivo for 11 days in three settings: basal conditions, exposure to AngII, and modulation of AngII effects by prior receptor blockade, i.e., AT1R, AT2R, and AT1R + AT2R. Tissue response was evaluated by combining mu CT and histological analysis. Basal-cultured femurs expressed components of RAS, namely ACE, AT1R, AT2R, and MasR (qPCR analysis). Bone formation occurred in the diaphyseal region in all conditions. In basal-cultured femurs, AT1R blocking increased Bone Surface/Bone Volume (BS/BV), whereas Bone Volume/Tissue Volume (BV/TV) decreased with AT2R or AT1R + AT2R blockade. Exposure to AngII greatly decreased BV/TV compared to basal conditions. Receptor blockade prior to AngII addition prevented this effect, i.e., AT1R blockade induced BV/TV, whereas blocking AT2R caused lower BV/TV increase but greater BS/BV; AT1R + AT2R blockade also improved BV/TV. Concluding, the embryonic chick femur model was sensitive to three relevant RAS research setups, proving its usefulness to address AngII/AT1R/AT2R axis in bone both in basal and activated conditions. (AU) | |
| Processo FAPESP: | 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica |
| Beneficiário: | Carlos Ferreira dos Santos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/19227-6 - Investigação da modulação da resposta inflamatória em células orais e imunológicas humanas por Ang II e Ang 1-7 |
| Beneficiário: | Thais Francini Garbieri |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 18/23934-2 - Papel da angiotensina ii e da angiotensina 1-7 no metabolismo de células ósseas humanas |
| Beneficiário: | Thais Francini Garbieri |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |