A possible influence of extracellular polysaccharides on the analysis of intracellular metabolites from Trichoderma harzianum grown under carbon-limited conditions

Autor(es)

Gelain, Lucas ^{[1, 2]} ; da Cruz Pradella, Jose Geraldo ^[3] ; da Costa, Aline Carvalho ^[2] ; van der Wielen, Luuk ^{[1, 4]} ; van Gulik, Walter M. ^[1]

Afiliação do(s) autor(es)

^[1] Delft Univ Technol, Dept Biotechnol, Van der Maasweg 9, NL-2629 HZ Delft - Netherlands

^[2] Univ Estadual Campinas, Sch Chem Engn, Av Albert Einstein 500, Campinas - Brazil

^[3] Univ Fed Sao Paulo, Inst Sci & Technol, Av Cesare Mansueto Giulio Lattes 1201, Sao Jose Dos Campos - Brazil

^[4] Univ Limerick, Bernal Inst, Limerick V94 T9PX - Ireland

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 125, n. 5, p. 368-377, MAY 2021.
• Citações Web of Science: 0

Resumo

Intracellular metabolites were evaluated during the continuous growth of Trichoderma harzianum P49P11 under carbon-limited conditions. Four different conditions in duplicate were investigated (10 and 20 g/L of glucose, 5.26/5.26 g/L of fructose/glucose and 10 g/L of sucrose in the feed). Differences in the values of some specific concentrations of intracellular metabolites were observed at steady-state for the duplicates. The presence of extracellular polysaccharide was confirmed in the supernatant of all conditions based on FT-IR and proton NMR. Fragments of polysaccharides from the cell wall could be released due to the shear stress and since the cells can consume them under carbon-limited conditions, this could create an unpredictable carbon flow rate into the cells. According to the values of the metabolite concentrations, it was considered that the consumption of those fragments was interfering with the analysis. (c) 2020 The Author(s). Published by Elsevier Ltd on behalf of British Mycological Society. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). (AU)

• Processo FAPESP: 14/22537-9 - Modelagem matemática da produção de enzimas pelo Trichoderma harzianum em fermentação submersa
• Beneficiário: Lucas Gelain
• Modalidade de apoio: Bolsas no Brasil - Doutorado