Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model

Autor(es)

Saraiva, M. M. S. ^[1] ; Rodrigues Alves, L. B. ^[1] ; Monte, D. F. M. ^[1] ; Ferreira, T. S. ^[1] ; Benevides, V. P. ^[1] ; Barbosa, F. O. ^[1] ; Freitas Neto, O. C. ^[2] ; Almeida, A. M. ^[1] ; Barrow, P. A. ^[3] ; Berchieri Junior, A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ FCAV Unesp, Lab Avian Pathol, Dept Pathol Theriogenol & One Hlth, Jaboticabal - Brazil

^[2] Fed Univ Minas Gerais UFMG, Vet Sch, Dept Prevent Vet Med, Belo Horizonte, MG - Brazil

^[3] Univ Surrey, Sch Vet Med & Sci, Guildford, Surrey - England

• Tipo de documento: Artigo Científico
• Fonte: AVIAN PATHOLOGY; v. 50, n. 3, p. 257-268, MAY 4 2021.
• Citações Web of Science: 0

Resumo

Salmonella enterica serovars use self-induced intestinal inflammation to increase electron acceptor availability and to obtain a growth advantage in the host gut. There is evidence suggesting that the ability of Salmonella to use tetrathionate and 1,2-propanediol provides an advantage in murine infection. Thus, we present here the first study to evaluate both systemic infection and faecal excretion in commercial poultry challenged by Salmonella Enteritidis (SE) and S. Typhimurium (STM) harbouring deletions in ttrA and pduA genes, which are crucial to the metabolism of tetrathionate and 1,2-propanediol, respectively. Mutant strains were excreted at higher rates when compared to the wild-type strains. The highest rates were observed with white egg-layer and brown egg-layer chicks (67.5%), and broiler chicks (56.7%) challenged by SE Delta ttrA Delta pduA, and brown egg-layer chicks (64.8%) challenged by STM Delta ttrA Delta pduA. SE Delta ttrA Delta pduA presented higher bacterial counts in the liver and spleen of the three chicken lineages and caecal contents from the broiler chickens, whereas STM Delta ttrA Delta pduA presented higher counts in the liver and spleen of the broiler and brown-egg chickens for 28 days post-infection (P < 0.05). The ttrA and pduA genes do not appear to be major virulence determinants in faecal excretion or invasiveness for SE and STM in chickens. (AU)

• Processo FAPESP: 20/06076-2 - Avaliação da infecção de aves (Gallus gallus domesticus) por Salmonella Enteritidis, Salmonella Typhimurium e Salmonella Heidelberg contendo deleção dos genes ttrA e pduA
• Beneficiário: Daniel Farias Marinho Do Monte
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/21301-2 - Avaliação da infecção de aves (Gallus gallus domesticus) por Salmonella enteritidis, Salmonella typhimurium e Salmonella heidelberg contendo deleção dos genes ttrA e pduA
• Beneficiário: Mauro de Mesquita Souza Saraiva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/03189-0 - Avaliação da infecção de aves (Gallus gallus domesticus) por Salmonella enteritidis, Salmonella typhimurium e Salmonella heidelberg contendo deleção dos genes ttrA e pduA
• Beneficiário: Angelo Berchieri Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático