New Role for a Commercially Available Bioinsecticide: Bacillus thuringiensis Berliner Biodegrades the Pyrethroid Cypermethrin

Autor(es)

Birolli, Willian Garcia ^[1] ; dos Santos, Alef ^[1] ; Pilau, Eduardo ^[2] ; Rodrigues-Filho, Edson ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Ctr Exact Sci & Technol, Lab Micromol Biochem Microorganisms LaBioMMi, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Maringa, Dept Chem, Lab Biomol & Mass Spectrometry, BR-87020080 Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science & Technology; v. 55, n. 8, p. 4792-4803, APR 20 2021.
• Citações Web of Science: 2

Resumo

The microbial diversity of several environments has been explored by researchers for the biodegradation of pyrethroids. In this study, a new approach was employed aiming at the use of Bacillus thuringiensis Berliner, a strain commercially available as bioinsecticide, for Cypermethrin (Cyp) biodegradation. This bacterial strain grew in the presence of Cyp and biodegraded this xenobiotic in a liquid medium. A central composite design for surface response methodology was employed for biodegradation. Under optimized conditions (50 mg.L-1 of Cyp, pH 8.5, 37 degrees C), 83.5% biodegradation was determined with the production of 12.0 +/- 0.6 mg.L-1 3-phenoxybenzoic acid after 5 days. Moreover, a biodegradation pathway with the 18 compounds identified by GC-MS and LC-MS/MS was proposed. Experiments in soil for 28 days at 30 degrees C were performed, and 16.7% Cyp degradation was determined under abiotic conditions, whereas 36.6 +/- 1.9% biodegradation was observed for B. thuringiensis Berliner with the native microbiome, indicating that bioaugmentation with this strain promoted a significant increase in the Cyp decontamination. Therefore, B. thuringiensis Berliner can act as biodegrader agent and insecticide at the same time, promoting decontamination of chemicals as Cyp while maintaining the protection of crops against insects. Moreover, B. thuringiensis species can produce bacteriocins with antifungal activity, which may increase agricultural productivity. (AU)

• Processo FAPESP: 16/09690-8 - Prospecção de micro-organismos endofíticos produtores de terpenóides
• Beneficiário: Edson Rodrigues Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/19721-0 - Emprego de microrganismos ambientais na biodegradação de agrotóxicos estrobilurinicos e piretróides amplamente utilizados na agricultura brasileira
• Beneficiário: Willian Garcia Birolli
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado