Comparative Genomics Supports That Brazilian Bioethanol Saccharomyces cerevisiae Comprise a Unified Group of Domesticated Strains Related to Cachaca Spirit Yeasts

Autor(es):Mostrar menos -

Jacobus, Ana Paula ^[1] ; Stephens, Timothy G. ^{[2, 3]} ; Youssef, Pierre ^[2] ; Gonzalez-Pech, Raul ^{[4, 2]} ; Ciccotosto-Camp, Michael M. ^{[5, 6]} ; Dougan, Katherine E. ^{[5, 6]} ; Chen, Yibi ^{[2, 5, 6]} ; Basso, Luiz Carlos ^[7] ; Frazzon, Jeverson ^[8] ; Chan, Cheong Xin ^{[2, 5, 6]} ; Gross, Jeferson ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Bioenergy Res, Lab Genom & Expt Evolut Yeasts, Rio Claro - Brazil

^[2] Univ Queensland, Inst Mol Biosci, Brisbane, Qld - Australia

^[3] Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ - USA

^[4] Univ S Florida, Dept Integrat Biol, Tampa, FL 33620 - USA

^[5] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld - Australia

^[6] Univ Queensland, Australian Ctr Ecogen, Brisbane, Qld - Australia

^[7] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Biol Sci, Piracicaba - Brazil

^[8] Fed Univ Rio Grande Sul UFRGS, Inst Food Sci & Technol, Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 12, APR 15 2021.
• Citações Web of Science: 1

Resumo

Ethanol production from sugarcane is a key renewable fuel industry in Brazil. Major drivers of this alcoholic fermentation are Saccharomyces cerevisiae strains that originally were contaminants to the system and yet prevail in the industrial process. Here we present newly sequenced genomes (using Illumina short-read and PacBio long-read data) of two monosporic isolates (H3 and H4) of the S. cerevisiae PE-2, a predominant bioethanol strain in Brazil. The assembled genomes of H3 and H4, together with 42 draft genomes of sugarcane-fermenting (fuel ethanol plus cachaca) strains, were compared against those of the reference S288C and diverse S. cerevisiae. All genomes of bioethanol yeasts have amplified SNO2(3)/SNZ2(3) gene clusters for vitamin B1/B6 biosynthesis, and display ubiquitous presence of a particular family of SAM-dependent methyl transferases, rare in S. cerevisiae. Widespread amplifications of quinone oxidoreductases YCR102C/YLR460C/YNL134C, and the structural or punctual variations among aquaporins and components of the iron homeostasis system, likely represent adaptations to industrial fermentation. Interesting is the pervasive presence among the bioethanol/cachaca strains of a five-gene cluster (Region B) that is a known phylogenetic signature of European wine yeasts. Combining genomes of H3, H4, and 195 yeast strains, we comprehensively assessed whole-genome phylogeny of these taxa using an alignment-free approach. The 197-genome phylogeny substantiates that bioethanol yeasts are monophyletic and closely related to the cachaca and wine strains. Our results support the hypothesis that biofuel-producing yeasts in Brazil may have been co-opted from a pool of yeasts that were pre-adapted to alcoholic fermentation of sugarcane for the distillation of cachaca spirit, which historically is a much older industry than the large-scale fuel ethanol production. (AU)

• Processo FAPESP: 17/24453-5 - Rede de colaboração: abordagens genéticas modernas para aumentar a tolerância de leveduras a hidrolisados lignocelulósicos enriquecidos
• Beneficiário: Ana Paula Jacobus
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 13/15743-9 - O impacto evolutivo da reprodução sexual ao longo de 5.000 gerações de leveduras e sua aplicação na engenharia de linhagens tolerantes ao etanol
• Beneficiário: Jeferson Gross
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 18/15159-9 - Abordagens genômicas integradas para o entendimento da tolerância ao estresse em leveduras produtoras de bioetanol e simbiontes de recifes de corais
• Beneficiário: Jeferson Gross
• Modalidade de apoio: Auxílio à Pesquisa - Regular