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Almeida, Caleo Panhoca de ^[1] ; de Carvalho Paulino, Jean Fausto ^[1] ; Bonfante, Gabriel Francesco Janini ^[1] ; Perseguini, Juliana Morini Kupper Cardoso ^[1] ; Santos, Isabella Laporte ^[1] ; Goncalves, Joao Guilherme Ribeiro ^[2] ; Patricio, Flavia Rodrigues Alves ^[3] ; Taniguti, Cristiane Hayumi ^[4] ; Gesteira, Gabriel de Siqueira ^[4] ; Garcia, Antonio Augusto Franco ^[4] ; Song, Qijian ^[5] ; Carbonell, Sergio Augusto Morais ^[2] ; Chiorato, Alisson Fernando ^[2] ; Benchimol-Reis, Luciana Lasry ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron IAC, Ctr Pesquisa Recursos Genet Vegetais, Campinas - Brazil

^[2] Inst Agron IAC, Ctr Graos & Fibras, Campinas - Brazil

^[3] Inst Biol IB, Lab Fitopatol, Campinas - Brazil

^[4] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil

^[5] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD - USA

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 12, APR 13 2021.
• Citações Web of Science: 4

Resumo

Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Studies based on different isolates and populations have already been carried out to elucidate the genetic mechanisms of resistance to ALS. However, understanding of the interaction of this resistance with the reproductive stages of common bean is lacking. The aim of the present study was to identify ALS resistance loci at different plant growth stages (PGS) by association and linkage mapping approaches. An BC2F3 inter-gene pool cross population (AND 277 x IAC-Milenio - AM population) profiled with 1,091 SNPs from genotyping by sequencing (GBS) was used for linkage mapping, and a carioca diversity panel (CDP) genotyped by 5,398 SNPs from BeadChip assay technology was used for association mapping. Both populations were evaluated for ALS resistance at the V2 and V3 PGSs (controlled conditions) and R8 PGS (field conditions). Different QTL (quantitative trait loci) were detected for the three PGSs and both populations, showing a different quantitative profile of the disease at different plant growth stages. For the three PGS, multiple interval mapping (MIM) identified seven significant QTL, and the Genome-wide association study (GWAS) identified fourteen associate SNPs. Several loci validated regions of previous studies, and Phg-1, Phg-2, Phg-4, and Phg-5, among the 5 loci of greatest effects reported in the literature, were detected in the CDP. The AND 277 cultivar contained both the Phg-1 and the Phg-5 QTL, which is reported for the first time in the descendant cultivar CAL143 as ALS10.1(UC). The novel QTL named ALS11.1(AM) was located at the beginning of chromosome Pv11. Gene annotation revealed several putative resistance genes involved in the ALS response at the three PGSs, and with the markers and loci identified, new specific molecular markers can be developed, representing a powerful tool for common bean crop improvement and for gain in ALS resistance. (AU)

• Processo FAPESP: 18/20992-1 - Estudo do controle genético do escurecimento tardio e de coloração do tegumento em grãos de feijão do tipo carioca
• Beneficiário: Cassia Cristina Augusto Pereira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/15526-1 - Estudo de associação genômica ampla para identificação de locus de interesse agronômico em feijão comum.
• Beneficiário: Caléo Panhoca de Almeida
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 19/19670-2 - Genômica populacional e GWAS para resistência à doenças e identificação de regiões de introgressão de pool andino em acessos de feijão carioca
• Beneficiário: Caléo Panhoca de Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/11145-2 - Seleção assistida por marcadores moleculares pelo método de AB-QTL e por genotipagem por sequenciamento (GBS) visando à resistência à mancha angular e antracnose em feijão comum
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/24711-4 - Estudo de associação ampla do genoma para resistência à Fusarium oxysporum f. sp. phaseoli e para cor de tegumento em feijoeiro comum
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/01753-3 - Seleção assistida por marcadores moleculares e genotipagem por sequenciamento (GBS) visando à resistência à mancha angular em feijão comum
• Beneficiário: Caléo Panhoca de Almeida
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/08280-5 - Seleção assistida por marcadores moleculares visando à resistência à mancha angular em feijoeiro
• Beneficiário: Juliana Morini Küpper Cardoso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado